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User:Wrigh312

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SUPER BOWL (Round 5)

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBACSU:YOKF2012-04-15 17:41:25 CDTGO:0004518 nuclease activity (F)PMID:11584000ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 5: DNase activity in cell-free extracts from wild-type and yokF deficient mutants exhibits nuclease activity.

challenge
acceptableECOLI:ZUPT2012-04-15 17:53:56 CDTGO:0006829 zinc ion transport (P)PMID:11790762ECO:0000315 mutant phenotype evidence used in manual assertion

Fig. 3: Induced expression of zupT in mutants lacking all zinc transporters yielded much greater uptake of zinc than the controls lacking transduction by the zupT vector.

challenge
updatedbyinstructorARATH:HMA3B2012-04-15 13:26:51 CDTGO:0071585 detoxification of cadmium ion (P)PMID:19036834ECO:0000315 mutant phenotype evidence used in manual assertion

Fig.5: Overexpressed HMA3 in plant protoplasts tagged with BTC-5N/AM (a Cd/Zn fluorescing probe) conferred much greater fluorescence (particularly in vacuoles) when treated with Cd than wild-type plants.

challenge
acceptableARATH:G2OX32012-04-15 15:22:00 CDTGO:0009686 gibberellin biosynthetic process (P)PMID:7604047ECO:0000314 direct assay evidence used in manual assertion

Fig. 4: GA 20-oxidase activity, present in steps of the gibberellin biosynthesis pathway

challenge
acceptableORYSJ:HAK12012-04-15 15:25:25 CDTGO:0006813 potassium ion transport (P)PMID:12376644ECO:0000314 direct assay evidence used in manual assertion

(Fig.7) K+ starved rice and yeast expressing OsHAK1 were grown on K+ containing media. The rate of depletion followed a Michaelis-Menten relationship in both rice and yeast mutants.

challenge

acceptable:4
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Wrigh312

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Wrigh312


MSU Internal

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
requireschangesARATH:AHM4B2012-04-01 14:49:02 CDTGO:0005773 vacuole (C)PMID:15013746ECO:0000269 experimental evidence used in manual assertion

GFP fluorescence used to visualize HMA3 localization. Only found at vacuole (Fig. 4)

challenge

acceptable:0
unacceptable:0
requires_changes:1
flagged:0

Annotations challenged by Wrigh312

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history

0 annotations fixed by Wrigh312