PMID:15013746

Citation	Gravot, A, Lieutaud, A, Verret, F, Auroy, P, Vavasseur, A and Richaud, P (2004) AtHMA3, a plant P1B-ATPase, functions as a Cd/Pb transporter in yeast. FEBS Lett. 561:22-8
Abstract	The Arabidopsis thaliana AtHMA3 protein belongs to the P(1B)-adenosine triphosphatase (ATPase) transporter family, involved in heavy metal transport. Functional expression of AtHMA3 phenotypically complements the Cd/Pb-hypersensitive yeast strain Deltaycf1, but not the Zn-hypersensitive mutant Deltazrc1. AtHMA3-complemented Deltaycf1 cells accumulate the same amount of cadmium as YCF1-complemented Deltaycf1 cells or wild-type cells, suggesting that AtHMA3 carries out an intracellular sequestration of Cd. A mutant of AtHMA3 altered in the P-ATPase phosphorylation domain did not complement Deltaycf1, suggesting that metal transport rather than chelation is involved. The fusion protein AtHMA3::green fluorescent protein (GFP) is localized at the vacuole, consistent with a role in the influx of cadmium into the vacuolar compartment. In A. thaliana, the mRNA of AtHMA3 was detected mainly in roots, old rosette leaves and cauline leaves. The expression levels were not affected by cadmium or zinc treatments.
Links	PubMed Online version:10.1016/S0014-5793(04)00072-9
Keywords	Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Arabidopsis Proteins/genetics; Arabidopsis Proteins/metabolism; Arabidopsis Proteins/physiology; Cadmium/metabolism; Lead/metabolism; Metals, Heavy/metabolism; Molecular Sequence Data; Plant Leaves/genetics; Plant Roots/genetics; RNA, Messenger/analysis; Sequence Alignment; Vacuoles/metabolism; Yeasts/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
ARATH:AHM4B		GO:0005773: vacuole	ECO:0000269:		C		GFP fluorescence used to visualize HMA3 localization. Only found at vacuole (Fig. 4)	complete CACAO 4202

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