CACAO Spring 2018
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
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| updatedbyinstructor | DROME:RB27C | 2018-04-13 19:13:25 CDT | GO:0030371 translation repressor activity (F) | PMID:29635389 | ECO:0000315 mutant phenotype evidence used in manual assertion | They depleted Hrp48 from SL2 cells and tested the ability of exogenous SXL to inhibit the translation of a reporter containing full-length msl-2 5′ and 3′ UTRs (Figure 3A). Depletion of Hrp48 indeed reduced the capacity of SXL to inhibit translation of the reporter (Figure 3B). The results of this paper support a model where a repressor complex composed of SXL, UNR and Hrp48 assembles at the 3′ UTR of msl-2 mRNA; Hrp48 then contributes to inhibit ribosome recruitment by targeting eIF3d.(Figure 8)
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| updatedbyinstructor | DROME:EI3D1 | 2018-04-12 21:08:46 CDT | GO:0003743 translation initiation factor activity (F) | PMID:29635389 | ECO:0000315 mutant phenotype evidence used in manual assertion | Depletion of eIF3d from SL2 cells reduced translation of an msl-2 reporter, while depletion of other subunits (eIF3e, eIF3h) had no effect (Figure 5DE)
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| updatedbyinstructor | HUMAN:DDX49 | 2018-04-22 14:05:33 CDT | GO:0030307 positive regulation of cell growth (P) | PMID:29618122 | ECO:0000315 mutant phenotype evidence used in manual assertion | HeLa cells were transfected with control siRNA, DDX49 siRNA, pEGFP-C1 vector or pEGFP-DDX49 construct as indicated and the cell numbers were counted at 48, 72 and 96 hours post-transfection. The colony forming potential was analyzed in cells treated with control siRNA, DDX49 siRNA, pEGFP-C1 vector or pEGFP-DDX49 construct as indicated(Figure 5C, D). They observed a very strong reduction in colony forming ability of DDX49 depleted cells suggesting that DDX49 is essential for proper cell growth and proliferation (Figure 5C)
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| acceptable | HUMAN:DDX49 | 2018-04-22 14:03:33 CDT | GO:0044357 regulation of rRNA stability (P) | PMID:29618122 | ECO:0000315 mutant phenotype evidence used in manual assertion | They assessed the stability of 47S rRNA by actinomycin D pulse chase assay and found that the siRNA mediated depletion of DDX49 substantially reduced the stability of 47S rRNA, while the overexpression of DDX49 did not show any alterations (Figure 4B). This suggests that DDX49 is required to stabilize the 47S rRNA.
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| acceptable | MOUSE:PTCD1 | 2018-04-22 19:30:36 CDT | GO:0032543 mitochondrial translation (P) | PMID:29617655 | ECO:0000315 mutant phenotype evidence used in manual assertion | In this paper, they measured de novo mitochondrial protein synthesis of the 13 mtDNA-encoded respiratory complex subunits and showed a dramatic loss of translation in the Ptcd1 knockout mice compared to controls in both tissues (Figures 3A and S1A), indicating that PTCD1 is essential for mitochondrial protein synthesis.
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Annotations challenged by Stavana
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
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