Spring 2012 Open Competition
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
| requireschanges | ECOLI:FTSZ | 2012-03-26 11:45:19 CDT | GO:0051301 cell division (P) | PMID:2045370 | ECO:0000315 mutant phenotype evidence used in manual assertion | Fig 3. The researcher constructed a null allele of ftsZ in a strain carrying additional copies offtsZ on a plasmid with a temperature-sensitive replication defect. This
strain was temperature sensitive for cell division and viability, confirming that ftsZ is an essential cell division
gene.
| challenge |
| requireschanges | ECOLI:FTSZ | 2012-03-26 11:49:53 CDT | GO:0003924 GTPase activity (F) | PMID:22056926 | ECO:0000315 mutant phenotype evidence used in manual assertion | FIG 2
Deletion of ZapB specifically reduces FtsZ division potential. Overnight cultures of TS mutant strains were grown to equal density in LB medium at 30°C and serially diluted (10−1 to 10−6) in LB medium. Aliquots (10 μl) were spotted on NA plates incubated at 30°C overnight.
| challenge |
| requireschanges | ECOLI:RS2 | 2012-03-26 12:31:18 CDT | GO:0003735 structural constituent of ribosome (F) | PMID:161328 | ECO:0000315 mutant phenotype evidence used in manual assertion | This mutation leads to thermosensitivity of growth and impaired in vivo assembly of 30 S ribosomal subunits at 42 °C. The strain carrying the mutation has an altered S2 ribosomal protein as judged by (1) its inability to maintain stable complex with the ribosome under mild washing conditions and (2) its altered electrophoretic mobility.
| challenge |
| acceptable | ECOLI:FTSN | 2012-03-26 12:47:27 CDT | GO:0051301 cell division (P) | PMID:8509333 | ECO:0000314 direct assay evidence used in manual assertion | | challenge |
| requireschanges | ECOLI:ACKA | 2012-04-12 21:59:21 CDT | GO:0008776 acetate kinase activity (F) | PMID:2536666 | ECO:0000315 mutant phenotype evidence used in manual assertion | | challenge |
| requireschanges | ECOLI:DSBA | 2012-04-13 15:18:10 CDT | GO:0006467 protein thiol-disulfide exchange (P) | PMID:1934062 | ECO:0000315 mutant phenotype evidence used in manual assertion | In dsbA mutant cells, pulse-labeled beta-lactamase, alkaline phosphatase, and OmpA are secreted but largely lack disulfide bonds.
| challenge |
| requireschanges | ECOLI:TRKG | 2012-04-13 16:42:59 CDT | GO:0015388 potassium uptake transmembrane transporter activity (F) | PMID:1987159 | ECO:0000315 mutant phenotype evidence used in manual assertion | The analysis of mutants of Escherichia coli that require elevated concentrations of K+ for growth has revealed two new genes, trkG, near minute 30 within the cryptic rac prophage, and trkH, near minute 87, the products of which affect constitutive K+ transport. The analysis of these and other trk mutations suggests that high rates of transport, previously considered to represent the activity of a single system, named TrkA, appear to be the sum of two systems, here named TrkG and TrkH.
| challenge |
| requireschanges | ECOLI:TRKH | 2012-04-13 16:49:35 CDT | GO:0006813 potassium ion transport (P) | PMID:1987159 | ECO:0000315 mutant phenotype evidence used in manual assertion | The analysis of mutants of Escherichia coli that require elevated concentrations of K+ for growth has revealed two new genes, trkG, near minute 30 within the cryptic rac prophage, and trkH, near minute 87, the products of which affect constitutive K+ transport. The analysis of these and other trk mutations suggests that high rates of transport, previously considered to represent the activity of a single system, named TrkA, appear to be the sum of two systems, here named TrkG and TrkH.
| challenge |
| requireschanges | ECOLI:SYA | 2012-04-14 16:40:02 CDT | GO:0006816 calcium ion transport (P) | PMID:383694 | ECO:0000315 mutant phenotype evidence used in manual assertion | Fig 1. Mutants sensitive to growth inhibition by CaCl2 were found to have alterations in calcium uptake in everted membrane vesicles. These mutations map at different loci on the Escherichia coli chromosomes. A mutation at the calA locus results in vesicles which have two- to threefold higher levels of uptake activity than vesicles from wild-type cells. The calA mutation is phenotypically expressed as increased sensitivity to CaCl2 in a strain also harboring a mutation in the corA locus, which is involved in Mg2+ transport.
| challenge |
acceptable:1
unacceptable:0
requires_changes:8
flagged:0
Annotations challenged by St413403
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by St413403
Internal Competition only involving UCF students
My Annotations
| Status | Page | Date/Time | GO Term (Aspect) | Reference | Evidence | Notes | Links |
|---|
acceptable:0
unacceptable:0
requires_changes:0
flagged:0
Annotations challenged by St413403
| Status | Author,Group | Page | GO Term (Aspect) | Reference | Evidence | Links | Page history |
|---|
0 annotations fixed by St413403
