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Bollen, A, Lathe, R, Herzog, A, Denicourt, D, Lecocq, JP, Desmarez, L and Lavallé, R (1979) A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB). J. Mol. Biol. 132:219-33
No abstract in PubMed
Bacteriophage lambda/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Genes; Genes, Lethal; Immunodiffusion; Lysogeny; Mutation; Protein Biosynthesis; Ribosomal Proteins/biosynthesis; Ribosomal Proteins/genetics; Species Specificity; Transcription, Genetic; Transduction, Genetic; beta-Galactosidase/biosynthesis
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0003735: structural constituent of ribosome||
This mutation leads to thermosensitivity of growth and impaired in vivo assembly of 30 S ribosomal subunits at 42 °C. The strain carrying the mutation has an altered S2 ribosomal protein as judged by (1) its inability to maintain stable complex with the ribosome under mild washing conditions and (2) its altered electrophoretic mobility.
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