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Category:Team James Watson

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableXANAC:Q8PHY8Ch009031, Team James Watson2017-02-08 13:40:01 CSTGO:0033554 cellular response to stress (P)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in table 4, after 20 min of

exposure to UV radiation, there were greater numbers of surviving cells of the wild-type strain than that of the gpsX mutant.

challenge
requireschangesPSEAE:Q9HUF2Ch009031, Team James Watson2012-04-15 12:02:11 CDTGO:0006935 chemotaxis (P)PMID:17521365ECO:0000315 mutant phenotype evidence used in manual assertion

Chemotaxis behavior was tested using casamino acids as the chemoattractant. As seen in figure 1 part f, the mutant showed a decreased response to chemoattractant compared with the wild type, suggesting that the PA5017 gene was involved (directly or indirectly) in chemotaxis.

challenge
requireschangesPSEAE:Q9HUF2Ch009031, Team James Watson2012-04-15 11:55:30 CDTGO:0071977 bacterial-type flagellar swimming motility (P)PMID:17521365ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure 1, part a through c, the PA5017 mutant displayed reduced swimming in medium containing 0.2% agar. The ability to swim decreased further as the concentration of agar increased and was lost in medium containing more than 0.3% agar. These results indicated that PA5017 was involved in flagellum-mediated motility. Figure 2 shows flagellum.

challenge
requireschangesPSEAE:Q9HUF2Ch009031, Team James Watson2012-04-15 11:55:30 CDTGO:0071978 bacterial-type flagellar swarming motility (P)PMID:17521365ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure 1, parts d and e, in the swarming assay, the wild-type PA68 strain exhibited a branching growth pattern away from the point of inoculation under all tested agar concentrations, while the PA5017 mutant displaced reduced swarming motility at a relatively low agar concentration (0.45%), and could not swarm at higher agar concentrations. These results indicated that PA5017 was involved in flagellum-mediated motility. Figure 2 shows flagellum.

challenge
requireschangesSTRMU:Q1LZ59Ch009031, Team James Watson2012-04-15 11:34:33 CDTGO:0042489 negative regulation of odontogenesis of dentin-containing tooth (P)PMID:16567561ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure B and D, dentinal (Dm) scores for the fruI−-infected group were 30–35% lower than those of the WT- or gtfA−-infected groups when the data was pooled for the two trials, mutation of fruI was associated with a loss of cariogenicity in dentin.

challenge
acceptableLACCA:PTLCBCh009031, Team James Watson2012-04-15 11:25:16 CDTGO:0046835 carbohydrate phosphorylation (P)PMID:2125053ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure 7, lanes 1 through 6, when each cysteine residue was replaced in turn by serine, lactose phosphorylation was found in mutants in which cysteine residues 31, 137, 152, 235, 316, or 349 had been replaced. As seen in figure 7, lane 7, no phosphorylation of the substrate occurred when Cys483 was replaced with a serine. As seen in figure 7, lanes 7 through 9, to confirm that this effect was not due to an undetected secondary mutation, a total of five independently obtained Cys483+Ser483 mutants, from two different experiments, were assayed. It was found in all cases that these mutants did not phosphorylate lactose. As seen in figure 7, lanes 10 through 11, when Cys483 was replaced with histidine, an amino acid found to be involved in all other phosphoryltransfer reactions of the PTS, or tyrosine, no lactose phosphorylation was observed. The loss of phosphorylating activity appeared to be specifically due to the exchange of Cys483 and not due to disruption of the tertiary structure of the protein. Cys483 is the active center phosphorylated amino acid residue in EIIlacL.casei.

challenge
requireschangesECOLI:SYASt413403, Team James Watson2012-04-14 16:40:02 CDTGO:0006816 calcium ion transport (P)PMID:383694ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 1. Mutants sensitive to growth inhibition by CaCl2 were found to have alterations in calcium uptake in everted membrane vesicles. These mutations map at different loci on the Escherichia coli chromosomes. A mutation at the calA locus results in vesicles which have two- to threefold higher levels of uptake activity than vesicles from wild-type cells. The calA mutation is phenotypically expressed as increased sensitivity to CaCl2 in a strain also harboring a mutation in the corA locus, which is involved in Mg2+ transport.

challenge
requireschangesXANAC:Q8PHY8Ch009031, Team James Watson2012-04-14 00:43:57 CDTGO:0009279 cell outer membrane (C)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 3B, a sodium dodecylsulphate-polyacrylamide gel electrophoresis analysis showed that LPS of the gpsX mutant was different from that of the wild-type strain 306. Two bands corresponding to the O-antigen containing LPS were completely lost in the gpsX mutant, compared to wild type strain 306.

challenge
requireschangesECOLI:TRKHSt413403, Team James Watson2012-04-13 16:49:35 CDTGO:0006813 potassium ion transport (P)PMID:1987159ECO:0000315 mutant phenotype evidence used in manual assertion

The analysis of mutants of Escherichia coli that require elevated concentrations of K+ for growth has revealed two new genes, trkG, near minute 30 within the cryptic rac prophage, and trkH, near minute 87, the products of which affect constitutive K+ transport. The analysis of these and other trk mutations suggests that high rates of transport, previously considered to represent the activity of a single system, named TrkA, appear to be the sum of two systems, here named TrkG and TrkH.

challenge
requireschangesECOLI:TRKGSt413403, Team James Watson2012-04-13 16:42:59 CDTGO:0015388 potassium uptake transmembrane transporter activity (F)PMID:1987159ECO:0000315 mutant phenotype evidence used in manual assertion

The analysis of mutants of Escherichia coli that require elevated concentrations of K+ for growth has revealed two new genes, trkG, near minute 30 within the cryptic rac prophage, and trkH, near minute 87, the products of which affect constitutive K+ transport. The analysis of these and other trk mutations suggests that high rates of transport, previously considered to represent the activity of a single system, named TrkA, appear to be the sum of two systems, here named TrkG and TrkH.

challenge
requireschangesECOLI:DSBASt413403, Team James Watson2012-04-13 15:18:10 CDTGO:0006467 protein thiol-disulfide exchange (P)PMID:1934062ECO:0000315 mutant phenotype evidence used in manual assertion

In dsbA mutant cells, pulse-labeled beta-lactamase, alkaline phosphatase, and OmpA are secreted but largely lack disulfide bonds.

challenge
requireschangesECOLI:ACKASt413403, Team James Watson2012-04-12 21:59:21 CDTGO:0008776 acetate kinase activity (F)PMID:2536666ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1

challenge
acceptableXANAC:Q8PHY8Ch009031, Team James Watson2012-04-11 22:59:36 CDTGO:0044407 single-species biofilm formation in or on host organism (P)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 6C, the gpsX mutant showed declined biofilm formation on citrus leaf surfaces as compared to the wild type.

challenge
acceptableXANAC:Q8PHY8Ch009031, Team James Watson2012-04-11 22:48:49 CDTGO:0044011 single-species biofilm formation on inanimate substrate (P)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 6A and 6B, the gpsX mutant was examined on polystyrene and glass surfaces. The gpsX mutant 223 G4 (gpsX-) exhibited a significant reduction in biofilm formation both on polystyrene surface and in glass tubes compared to that of the wild-type, where the level of biofilm formation were approximately 30% and 40% of the wild-type level.

challenge
requireschangesXANAC:Q8PHY8Ch009031, Team James Watson2012-04-11 22:34:02 CDTGO:0048870 cell motility (P)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

As seen in figure 7, the gpsX mutant was evaluated for the mobile ability on swimming plates. The results showed that a significant reduction in swimming motility was observed in the gpsX mutant 223 G4 (gpsX-), compared with the wildtype strain.

challenge
acceptableXANAC:Q8PHY8Ch009031, Team James Watson2012-04-11 22:26:37 CDTGO:0009405 pathogenesis (P)PMID:22404966ECO:0000315 mutant phenotype evidence used in manual assertion

Xanthomonas axonopodis Glycosyltransferase (gpsX)

The virulence of the gpsX mutant was assessed on the host plant grapefruit using two inoculation methods: pressure infiltration and spray. As seen in figure 4A, upon infiltration at a concentration 105 colony-forming units (cfu)/ml the gpsX mutant induced less canker lesions than the wild-type strain 306 at 14 dpi. When infiltrated at a higher concentration (108 cfu/ml), the gpsX mutant induced significantly less lesions than wild type at 7 dpi, but caused similar disease symptoms as wild type at 14 dpi. Plant inoculation by spray showed that the gpsX mutant was reduced in virulence on grapefruit compared with the wild-type strain 306. After 21 days post inoculation the number of canker lesions on leaves infected with the gpsX mutant was significantly less than that inoculated with wild type strain.

challenge
acceptableSTAAU:G0Z026Ch009031, Team James Watson2012-04-01 16:25:50 CDTGO:0009405 pathogenesis (P)PMID:22022262ECO:0000315 mutant phenotype evidence used in manual assertion

The experiment compared the ability of wild type, mutant and repaired strains to cause lethal necrotizing pneumonia in a rabbit model. As seen in figure 6c, animals receiving wild type LAC, all 11 rabbits succumbed within 4 days, compared to 2 of 11 receiving LAC Δselx. Importantly, 4 of 4 rabbits infected with the repaired strain LAC Δselx rep succumbed within 4 days. As seen in figure 6d, body temperatures were recorded in the first 24 h of the experiment, and animals receiving strain LAC demonstrated significantly higher body temperatures than animals receiving the SElX-deficient strain LAC Δselx and LAC Δselx rep had wild type levels of pyrogenicity.

challenge
acceptableCLOBH:DNAKCh009031, Team James Watson2012-03-31 16:19:55 CDTGO:0034605 cellular response to heat (P)PMID:21378058ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure 6 and table 3, there was a large reduction in growth in the DnaK mutant strain of Clostridium botulinum as compared to the wild type strain of Clostridium botulinum when put under the stress of being grown at high temperatures (temperatures greater than 37°C). Also, as seen in figure 7, the DnaK mutant strain of Clostridium botulinum showed much less growth when put under a temperature gradient of 42 to 48°C when grown on TPGY plates.

challenge
acceptableCLOBH:DNAKCh009031, Team James Watson2012-03-31 16:15:24 CDTGO:0071468 cellular response to acidity (P)PMID:21378058ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in table 3 and in figure 6, there was a reduction in growth in the DnaK mutant strain of Clostridium botulinum as compared to the wild type strain of Clostridium botulinum when put under the stress of being grown at a pH of 5 or 6.

challenge
acceptableCLOBH:DNAKCh009031, Team James Watson2012-03-31 16:10:34 CDTGO:0071475 cellular hyperosmotic salinity response (P)PMID:21378058ECO:0000315 mutant phenotype evidence used in manual assertion

As seen in figure 6 and table 3, there was a large reduction in growth in the DnaK mutant strain of Clostridium botulinum as compared to the wild type strain of Clostridium botulinum when grown in both 3% and 3.5% NaCl concentrations.

challenge
acceptableBOVIN:ITPR3Ch009031, Team James Watson2012-03-29 16:05:37 CDTGO:0005791 rough endoplasmic reticulum (C)PMID:11584008ECO:0000314 direct assay evidence used in manual assertion

The bovine adrenal medullary chromaffin

cells were immunolabeled for type 1, 2, and 3 IP3R (10 nm) with each isoform-specific affinity-purified IP3R antibodies. As seen in figure 2C, the gold particles are also localized in the rough endoplasmic reticulum (rer as seen in the picture).

challenge
acceptableBOVIN:ITPR1Ch009031, Team James Watson2012-03-29 16:05:34 CDTGO:0005791 rough endoplasmic reticulum (C)PMID:11584008ECO:0000314 direct assay evidence used in manual assertion

The bovine adrenal medullary chromaffin

cells were immunolabeled for type 1, 2, and 3 IP3R (10 nm) with each isoform-specific affinity-purified IP3R antibodies. As seen in figure 2A, the gold particles are also localized in the rough endoplasmic reticulum (rer as seen in the picture).

challenge
acceptableBOVIN:ITPR2Ch009031, Team James Watson2012-03-29 16:03:17 CDTGO:0005791 rough endoplasmic reticulum (C)PMID:11584008ECO:0000314 direct assay evidence used in manual assertion

The bovine adrenal medullary chromaffin

cells were immunolabeled for type 1, 2, and 3 IP3R (10 nm) with each isoform-specific affinity-purified IP3R antibodies. As seen in figure 2B, the gold particles are also localized in the rough endoplasmic reticulum (rer as seen in the picture).

challenge
acceptableRICPR:TLCECh009031, Team James Watson2012-03-26 19:35:10 CDTGO:0015931 nucleobase-containing compound transport (P)PMID:16923893ECO:0000314 direct assay evidence used in manual assertion

The Tlc5 gene was cloned into the pET-11a expression vector and introduced into the C41 strain of E. coli by electroporation. Using filtration uptake assays, it was shown, as seen in figure 2B, that Tlc5 was able to transport GTP and GDP with very similar maximum rates in the E. coli expression vector. It can also be seen in chart 3 that the only compounds that demonstrated substantial inhibition of GTP transport when present at a 10-fold excess were GTP and GDP.

challenge
acceptableRICPR:TLCDCh009031, Team James Watson2012-03-26 19:33:29 CDTGO:0015931 nucleobase-containing compound transport (P)PMID:16923893ECO:0000314 direct assay evidence used in manual assertion

The Tlc4 gene was cloned into the pET-11a expression vector and introduced into the C41 strain of E. coli by electroporation. Using filtration uptake assays, it was shown, as seen in figure 2A, that Tlc4 was able to transport CTP, UTP, and GDP with very similar maximum rates in the E. coli expression vector. CTP (.1 mM) bound the most avidly followed by UTP (.3 mM) and then GDP (.6 mM).

challenge
acceptableRICPR:TLCACh009031, Team James Watson2012-03-26 19:21:37 CDTGO:0015867 ATP transport (P)PMID:16923893ECO:0000314 direct assay evidence used in manual assertion

Tlc1 homologue was cloned into the pET-

11a expression vector and introduced into the C41 strain of E. coli by electroporation. Figure 1 shows ATP being able to be transported into the cell as measured by a filtration uptake assay.

challenge
acceptableECOLI:FTSNSt413403, Team James Watson2012-03-26 12:47:27 CDTGO:0051301 cell division (P)PMID:8509333ECO:0000314 direct assay evidence used in manual assertion

Fig 3

challenge
requireschangesECOLI:RS2St413403, Team James Watson2012-03-26 12:31:18 CDTGO:0003735 structural constituent of ribosome (F)PMID:161328ECO:0000315 mutant phenotype evidence used in manual assertion

This mutation leads to thermosensitivity of growth and impaired in vivo assembly of 30 S ribosomal subunits at 42 °C. The strain carrying the mutation has an altered S2 ribosomal protein as judged by (1) its inability to maintain stable complex with the ribosome under mild washing conditions and (2) its altered electrophoretic mobility.

challenge
requireschangesECOLI:FTSZSt413403, Team James Watson2012-03-26 11:49:53 CDTGO:0003924 GTPase activity (F)PMID:22056926ECO:0000315 mutant phenotype evidence used in manual assertion

FIG 2

Deletion of ZapB specifically reduces FtsZ division potential. Overnight cultures of TS mutant strains were grown to equal density in LB medium at 30°C and serially diluted (10−1 to 10−6) in LB medium. Aliquots (10 μl) were spotted on NA plates incubated at 30°C overnight.

challenge
requireschangesECOLI:FTSZSt413403, Team James Watson2012-03-26 11:45:19 CDTGO:0051301 cell division (P)PMID:2045370ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3. The researcher constructed a null allele of ftsZ in a strain carrying additional copies offtsZ on a plasmid with a temperature-sensitive replication defect. This

strain was temperature sensitive for cell division and viability, confirming that ftsZ is an essential cell division gene.

challenge

Pages in category "Team James Watson"

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