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User:Smahoney2

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableVIBF1:Q5E6142013-09-15 11:14:10 CDTGO:0008218 bioluminescence (P)PMID:14507383ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 3, ainS mutant failed to persist in the squid light organ.

challenge
acceptableVIBF1:Q5E6B42013-09-29 16:07:35 CDTGO:0008218 bioluminescence (P)PMID:14507383ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 3, the luxO mutant resulted in an acceleration of the onset of luminescence in culture

challenge
updatedbyinstructorECOLI:NUSA2013-10-08 22:36:32 CDTGO:0051259 protein complex oligomerization (P)PMID:23907089ECO:0000314 direct assay evidence used in manual assertion

In Figure 1, it is shown that under heat shock conditions NusA can form stable high molecular weight(HMW)oligomers.

challenge
unacceptableSALT1:D0ZSD62013-11-04 11:28:04 CSTGO:0043190 ATP-binding cassette (ABC) transporter complex (C)PMID:24169575ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 4, ΔmacAB mutants rapidly lost viability in the presence of 1 mM H2O2 as compared to wild-type Salmonella, suggesting MacAB is required for resistance to hydrogen peroxide in vitro.

challenge
unacceptableSALT1:D0ZSD62013-11-04 11:38:36 CSTGO:0043190 ATP-binding cassette (ABC) transporter complex (C)PMID:24169575ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 5, wild-type Salmonella transcomplements hydrogen peroxide sensitivity of the macAB mutant

challenge
acceptableVIBF1:Q5E2S42013-11-04 11:57:05 CSTGO:0008218 bioluminescence (P)PMID:12100554ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 5, V. fischeri strain PMF8, in which litR was insertionally inactivated, showed delayed onset of luminescence induction and emitted only about 20% as much light per cell as its parent.

challenge
acceptableVIBHA:LUXO2013-11-10 16:09:08 CSTGO:0008218 bioluminescence (P)PMID:12675810ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 1, the intensity of luminescence was higher in luxO– mutant than in wild-type cells early in growth

challenge
unacceptableVIBHA:LUXO2013-11-10 16:18:27 CSTGO:0008218 bioluminescence (P)PMID:12675810ECO:0000315 mutant phenotype evidence used in manual assertion

In Figure 4, the presence of luxRvh strongly stimulated luminescence in the V. harveyi luxO null mutant

challenge

acceptable:4
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Smahoney2

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorDbilling,
Team Green Machine
BACTK:B5SRV2GO:0003867 - obsolete 4-aminobutyrate transaminase activity (F)PMID:19854901ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

fixed by Smahoney2

1 annotations fixed by Smahoney2