User:Ramaswamya

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	BPSPP:O48472	2017-04-12 09:31:11 CDT	GO:0020002 host cell plasma membrane (C)	PMID:27825035	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 shows a SDS-Page analysis of the two gp24.1 and gp 26. The bands for gp 24.1 are shown under the Membrane section of the plate signifying that this gene is a membrane localized gene.	challenge
flagged	BPA18:HOLI	2017-04-12 10:17:18 CDT	GO:0019835 cytolysis (P)	PMID:12657053	ECO:0000315 mutant phenotype evidence used in manual assertion	Holin Annotation Notes: Hol 118(83) is a naturally occurring altered portion of the Holin gene “Hol118” which affects cell lysis timings. Hol118(83) had a truncated N-Terminal (Thus a different start codon then usual) and when translated this portion showed slowed cell lysis. The figure shows decrease in optical density for the Hol118(83) mutant phenotypes. The mutant phenotypes were replaced with different start codons which allowed for an increase rate of cell lysis as compared with the wild type Hol118(83). The actual function of Hol118 in the holin polypeptide chain is to increase the rate of cell lysis so by induction the portion of the N-terminal that was missing in the Hol118(83) seemed to counteract its effects and allow for normal functioning .In conclusion Hol118(83) was a strong antiholin that was already present in the holin genome which could be why in UniProt we see both Holin and Antiholin functions.	challenge
requireschanges	9CAUD:H2D0G4	2017-04-12 10:38:21 CDT	GO:0009253 peptidoglycan catabolic process (P)	PMID:22289622	ECO:0000314 direct assay evidence used in manual assertion	Figure 5 shows a line (A) and histogram(B) that plots the peptidoglycan degradation in Ecoli in response to the introduction of SPN1S endolysin into the sample. The endolysin seems to cleave the glycosydic linkage between the peptidoglycan molecules to reduce the overall optical density.	challenge

acceptable:0
unacceptable:1
requires_changes:1
flagged:1

Annotations challenged by Ramaswamya

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Mcgillje, Team Phergalicious	BPSPP:O48472	GO:0020002 - host cell plasma membrane (C)	PMID:27825035	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Ramaswamya

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