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Phage Hunters Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableBPSPP:O484722017-04-12 09:31:11 CDTGO:0020002 host cell plasma membrane (C)PMID:27825035ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows a SDS-Page analysis of the two gp24.1 and gp 26. The bands for gp 24.1 are shown under the Membrane section of the plate signifying that this gene is a membrane localized gene.

flaggedBPA18:HOLI2017-04-12 10:17:18 CDTGO:0019835 cytolysis (P)PMID:12657053ECO:0000315 mutant phenotype evidence used in manual assertion

Holin Annotation Notes:

Hol 118(83) is a naturally occurring altered portion of the Holin gene “Hol118” which affects cell lysis timings. Hol118(83) had a truncated N-Terminal (Thus a different start codon then usual) and when translated this portion showed slowed cell lysis. The figure shows decrease in optical density for the Hol118(83) mutant phenotypes. The mutant phenotypes were replaced with different start codons which allowed for an increase rate of cell lysis as compared with the wild type Hol118(83). The actual function of Hol118 in the holin polypeptide chain is to increase the rate of cell lysis so by induction the portion of the N-terminal that was missing in the Hol118(83) seemed to counteract its effects and allow for normal functioning .In conclusion Hol118(83) was a strong antiholin that was already present in the holin genome which could be why in UniProt we see both Holin and Antiholin functions.

requireschanges9CAUD:H2D0G42017-04-12 10:38:21 CDTGO:0009253 peptidoglycan catabolic process (P)PMID:22289622ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows a line (A) and histogram(B) that plots the peptidoglycan degradation in Ecoli in response to the introduction of SPN1S endolysin into the sample. The endolysin seems to cleave the glycosydic linkage between the peptidoglycan molecules to reduce the overall optical density.



Annotations challenged by Ramaswamya

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
Team Phergalicious
BPSPP:O48472GO:0020002 - host cell plasma membrane (C)PMID:27825035ECO:0000314 direct assay evidence used in manual assertionchallengeC: 2

0 annotations fixed by Ramaswamya

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