User:RajivRangan

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	BPT7:GP73	2016-04-18 06:00:55 CDT	GO:0019012 virion (C)	PMID:16054667	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3	challenge
updatedbyinstructor	BPT7:GP67	2016-04-18 06:06:39 CDT	GO:0039620 T=7 icosahedral viral capsid (C)	PMID:16054667	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig 3	challenge
unacceptable	BPT7:CAPSA	2016-04-26 12:30:01 CDT	GO:0098017 viral capsid, major subunit (C)	PMID:20962334	ECO:0000314 direct assay evidence used in manual assertion	Figure 2A: Researchers purified T7 virions from E.coli cells and separated the viral heads. Cryo-electron microscopy was used to examine the empty viral heads, and the structure of gp10A was determined to be that of a T=7 icosahedral capsid, major subunit.	challenge
updatedbyinstructor	BPT7:GP04	2016-04-26 21:27:53 CDT	GO:0044865 negative regulation by virus of host cell division (P)	PMID:24218612	ECO:0000314 direct assay evidence used in manual assertion	Figs 3,4	challenge
unacceptable	BPT7:DNBI	2016-05-01 19:40:34 CDT	GO:0003674 molecular_function (F)	PMID:1634538	ECO:0000314 direct assay evidence used in manual assertion	Figure 3: A nitrocellulose filter was used to determine levels of binding between gp2.5 and radioactively labeled DNA. Nitrocellulose is negatively charged, so only DNA bound to gp2.5 could be retained. With increasing concentrations of gp2.5, greater amounts of single-stranded DNA were retained. The experiment only demonstrated a small amount of binding between gp2.5 and double stranded DNA. This suggests that gp2.5's molecular function is the binding of ssDNA.	challenge
updatedbyinstructor	BPT3:RPOL	2016-05-01 20:37:50 CDT	GO:0003899 DNA-directed RNA polymerase activity (F)	PMID:3011596	ECO:0000314 direct assay evidence used in manual assertion	Fig 5. The T3 gene 1.0 was over-expressed on a plasmid, and the protein product was collected. T3 mutants with no functional gene 1.0 were incapable of initiating transcription, but regained function when in the presence of collected gp1.0 protein. T7 phages without a functional copy of gene 1.0 were not affected by phage T3 gp1.0. This suggests that the T3 gene 1.0 is a T3-specific RNA polymerase.	challenge
unacceptable	BPT7:RPOL	2016-05-01 20:57:10 CDT	GO:0034062 RNA polymerase activity (F)	PMID:6093820	ECO:0000314 direct assay evidence used in manual assertion	Gene 1.0 of phage T7 was cloned into and expressed on a plasmid vector. The protein produced from the expression of gene 1.0 was demonstrated to be a T7 RNA polymerase due to its ability to transcribe RNA from T7 DNA, when in the presence of rifampicin (T7 RNA polymerase is known to be unaffected by rifampicin, which is an antibiotic that inhibits bacterial RNA polymerase).	challenge

acceptable:0
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by RajivRangan

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

0 annotations fixed by RajivRangan

What do the icons mean in the status column?