PMID:24218612

Citation	Kiro, R, Molshanski-Mor, S, Yosef, I, Milam, SL, Erickson, HP and Qimron, U (2013) Gene product 0.4 increases bacteriophage T7 competitiveness by inhibiting host cell division. Proc. Natl. Acad. Sci. U.S.A. 110:19549-54
Abstract	Bacteriophages take over host resources primarily via the activity of proteins expressed early in infection. One of these proteins, produced by the Escherichia coli phage T7, is gene product (Gp) 0.4. Here, we show that Gp0.4 is a direct inhibitor of the E. coli filamenting temperature-sensitive mutant Z division protein. A chemically synthesized Gp0.4 binds to purified filamenting temperature-sensitive mutant Z protein and directly inhibits its assembly in vitro. Consequently, expression of Gp0.4 in vivo is lethal to E. coli and results in bacteria that are morphologically elongated. We further show that this inhibition of cell division by Gp0.4 enhances the bacteriophage's competitive ability. This division inhibition is thus a fascinating example of a strategy in bacteriophages to maximize utilization of their hosts' cell resources.
Links	PubMed PMC3845191 Online version:10.1073/pnas.1314096110
Keywords	Adaptation, Biological/genetics; Bacterial Proteins/genetics; Bacteriophage T7/genetics; Blotting, Western; Cytoskeletal Proteins/antagonists & inhibitors; Cytoskeletal Proteins/genetics; Escherichia coli/cytology; Escherichia coli/virology; Plasmids/genetics; Viral Proteins/genetics; Viral Proteins/metabolism; Viral Proteins/pharmacology
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BPT7:GP04		GO:0044865: negative regulation by virus of host cell division	ECO:0000314:		P		Figs 3,4	complete CACAO 11946

References

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