PMID:6093820

Citation	Grachev, MA and Pletnev, AG (1984) [Phage T7 RNA-polymerase: gene cloning and its structure]. Bioorg. Khim. 10:824-43
Abstract	Gene 1.0 to T7 phage coding for a phage-specific RNA polymerase has been cloned in a plasmid vector. One of the clones obtained, viz. E. coli K12 HB101 (pSK-T7-1.0a), produced an active T7 RNA polymerase as revealed by the fact that its extract stimulated RNA synthesis on the T7 DNA template in the presence of rifampicin. Analysis of gene 1.0 fragments isolated from pSK-T7-1.0a plasmid made it possible to refine the sequence of this gene which has been recently published by the authors of the present paper and, independently, by other workers. Another plasmid, pSK-T7-1.0b, did not differ from pSK-T7-1.0a in the restriction map. However, it failed to induce production of a rifampicin-insensitive RNA polymerase. Sequencing revealed a deletion of one T residue in gene 1.0 present in this plasmid.
Links	PubMed
Keywords	Amino Acid Sequence; Autoradiography; Base Sequence; Chromosome Mapping; Cloning, Molecular; DNA Restriction Enzymes; DNA, Viral/genetics; DNA-Directed RNA Polymerases/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Genes, Viral; Plasmids; T-Phages/enzymology; T-Phages/genetics
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Significance

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BPT7:RPOL		GO:0034062: RNA polymerase activity	ECO:0000314:		F		Gene 1.0 of phage T7 was cloned into and expressed on a plasmid vector. The protein produced from the expression of gene 1.0 was demonstrated to be a T7 RNA polymerase due to its ability to transcribe RNA from T7 DNA, when in the presence of rifampicin (T7 RNA polymerase is known to be unaffected by rifampicin, which is an antibiotic that inhibits bacterial RNA polymerase).	complete CACAO 12010

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