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BPT7:RPOL

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Species (Taxon ID) Enterobacteria phage T7 (Bacteriophage T7). (10760)
Gene Name(s) No Information Provided.
Protein Name(s) T7 RNA polymerase

DNA-directed RNA polymerase

External Links
UniProt P00573
EMBL V01146
V01127
M38308
PIR A94615
RefSeq NP_041960.1
PDB 1ARO
1CEZ
1H38
1MSW
1QLN
1S0V
1S76
1S77
2PI4
2PI5
3E2E
3E3J
4RNP
PDBsum 1ARO
1CEZ
1H38
1MSW
1QLN
1S0V
1S76
1S77
2PI4
2PI5
3E2E
3E3J
4RNP
ProteinModelPortal P00573
SMR P00573
DIP DIP-6091N
IntAct P00573
MINT MINT-1513587
BindingDB P00573
ChEMBL CHEMBL1075072
PRIDE P00573
GeneID 1261050
EvolutionaryTrace P00573
PMAP-CutDB P00573
Proteomes UP000000840
GO GO:0003677
GO:0003899
GO:0006351
Gene3D 1.10.1320.10
1.10.287.260
InterPro IPR024075
IPR002092
IPR029262
PANTHER PTHR10102
Pfam PF00940
PF14700
PROSITE PS00900
PS00489

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status

involved_in

GO:0039695

DNA-templated viral transcription

PMID:4580764[1]

ECO:0000314

direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002092

F

Seeded From UniProt

complete

enables

GO:0003899

DNA-directed 5'-3' RNA polymerase activity

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002092
InterPro:IPR024075

F

Seeded From UniProt

complete

involved_in

GO:0006351

transcription, DNA-templated

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR002092

P

Seeded From UniProt

complete

enables

GO:0003899

DNA-directed 5'-3' RNA polymerase activity

GO_REF:0000003

ECO:0000501

evidence used in automatic assertion

EC:2.7.7.6

F

Seeded From UniProt

complete

involved_in

GO:0019083

viral transcription

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-1195

P

Seeded From UniProt

complete

enables

GO:0016740

transferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0808

F

Seeded From UniProt

complete

enables

GO:0003899

DNA-directed 5'-3' RNA polymerase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0240

F

Seeded From UniProt

complete

enables

GO:0016779

nucleotidyltransferase activity

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0548

F

Seeded From UniProt

complete

GO:0034062

RNA polymerase activity

PMID:6093820[2]

ECO:0000314

F

Gene 1.0 of phage T7 was cloned into and expressed on a plasmid vector. The protein produced from the expression of gene 1.0 was demonstrated to be a T7 RNA polymerase due to its ability to transcribe RNA from T7 DNA, when in the presence of rifampicin (T7 RNA polymerase is known to be unaffected by rifampicin, which is an antibiotic that inhibits bacterial RNA polymerase).

complete
CACAO 12010

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Lillehaug, JR et al. (1973) In vivo and in vitro transcription of T7 early genes by T7 RNA polymerase. FEBS Lett. 33 335-8 PubMed GONUTS page
  2. Grachev, MA & Pletnev, AG (1984) [Phage T7 RNA-polymerase: gene cloning and its structure]. Bioorg. Khim. 10 824-43 PubMed GONUTS page