User:Kbohning

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	PSEAE:LASR	2013-09-25 14:13:36 CDT	GO:0003700 sequence-specific DNA binding transcription factor activity (F)	PMID:23066081	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1.section 3 shows the mutant strain in the Lux R protein and what happens in the presence of adenosine.	challenge
acceptable	9FLAV:G4XII4	2013-09-26 18:50:28 CDT	GO:0019079 viral genome replication (P)	PMID:22796133	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 2. Mutations is the NS5 protein in viral replication of Tick-borne encephalitis	challenge
acceptable	9FLAV:G4XII4	2013-09-26 18:56:55 CDT	GO:0019079 viral genome replication (P)	PMID:22796133	ECO:0000314 direct assay evidence used in manual assertion	Figure 3.A. Contains SDS-PAGE of cytoplasmic fractions. The NS5 specific antibody revealed a band at the expected size of TBEVNS5 (103 kDa), but also an additional band around 70 kDa.	challenge
unacceptable	9FLAV:G4XII4	2013-09-26 19:03:32 CDT	GO:0019079 viral genome replication (P)	PMID:22796133	ECO:0000314 direct assay evidence used in manual assertion	Figure 3B.Binding capacities of NS5 of Tick-borne encephalitis and West Nile virus encephalitis.	challenge
unacceptable	BACSU:TILS	2013-10-13 17:21:16 CDT	GO:0016563 transcription activator activity (F)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 4. SDS Page Analysis of co-purification of TilS and HprT by Ni-NTA affinity chromatography.	challenge
unacceptable	BACSU:TILS	2013-10-13 17:26:30 CDT	GO:0016563 transcription activator activity (F)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Fig. 5 Gel filtration chromatograms for His-tagged TilS alone.	challenge
unacceptable	BACSU:TILS	2013-10-13 17:28:52 CDT	GO:0016563 transcription activator activity (F)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Fig.7 In vitro transcription analyses.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:10:52 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 1A.Purification of NS5-interacting proteins using the TAP method	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:12:42 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 1B. Western Blot analysis of the purified proteins. Anti-Flag antibody was used to detect Flag-HA-NS5 protein. In lane 1 of both panels, the black arrowheads mark the expected full length Flag-HA-NS5.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:15:55 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 2A. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Hsp70-Myc.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:20:33 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 2C. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Ran-Myc.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:23:33 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 2B.Interaction of the JEV NS5 protein with identified cellular proteins. 293T cells were transfected with expression plasmids encoding Flag-NS5 and eEF-1α-Myc.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:25:17 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3B.Interaction of Hsp70 with deletion mutants of NS5 protein. Hsp70-Myc and a series of deletion mutants of Flag-NS5 were co-transfected in 293T cells, precipitated with mouse anti-Flag MAb and then subjected to Western blotting.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:26:37 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3C.The purified proteins of GST-NS5 (406-905) and His-Hsp70 were analyzed by SDS-PAGE.	challenge
requireschanges	9FLAV:Q9J1B7	2013-10-27 14:28:17 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 3D.The direct interaction between GST-NS5 RdRp domain and His-Hsp70 was analyzed by GST pull-down assay and Western blotting.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:31:47 CDT		PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 4A. Interaction of Hsp70 with components of the JEV replicase complex. Interaction of Hsp70 with JEV NS3. 293T cells were co-transfected with Myc-tagged Hsp70 plasmid and Flag-tagged NS3 plasmid or vector. Cell lysates were harvested 36 h after transfection for co-IP experiments with anti-Flag antibody. The precipitates were then analyzed by Western blotting with anti-Flag or anti-Myc antibody.	challenge
unacceptable	9FLAV:Q9J1B7	2013-10-27 14:33:02 CDT	GO:0008166 viral replication (P)	PMID:24086464	ECO:0000314 direct assay evidence used in manual assertion	Figure 4 (B) Association of Hsp70 with NS3 and NS5 proteins and viral RNA in JEV-infected cells. 293T cells were transfected with Hsp70-Myc expressing plasmid or vector before being mock-infected or infected with JEV at an MOI of 1.0. Cell lysates were harvested at 36 h p.i. and subjected to immunoprecipitation with antibody against Myc, followed by Western blotting for the detection of NS3 and NS5. The RNA were extracted from the precipitates and then subjected to RT-PCR for detecting viral RNA. RNA extracted from cells infected and uninfected with JEV was used as positive control (PC) and negative control (NC) respectively.	challenge

acceptable:2
unacceptable:14
requires_changes:1
flagged:0

Annotations challenged by Kbohning

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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