9FLAV:Q9J1B7

Species (Taxon ID)	Japanese encephalitis virus. (11072)
Gene Name(s)	NS5 (ECO:0000313 with EMBL:AAF63211.1)
Protein Name(s)	NS5 non-structural protein (ECO:0000313 with EMBL:AAF63211.1)
External Links
UniProt	Q9J1B7
EMBL	AF218068
GO	GO:0005524 GO:0003968
InterPro	IPR000208
Pfam	PF00972

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 1A.Purification of NS5-interacting proteins using the TAP method	complete CACAO 8945
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 1B. Western Blot analysis of the purified proteins. Anti-Flag antibody was used to detect Flag-HA-NS5 protein. In lane 1 of both panels, the black arrowheads mark the expected full length Flag-HA-NS5.	complete CACAO 8946
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 2A. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Hsp70-Myc.	complete CACAO 8947
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 2C. Interaction of the JEV NS5 protein with identified cellular proteins.293T cells were transfected with expression plasmids encoding Flag-NS5 and Ran-Myc.	complete CACAO 8948
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 2B.Interaction of the JEV NS5 protein with identified cellular proteins. 293T cells were transfected with expression plasmids encoding Flag-NS5 and eEF-1α-Myc.	complete CACAO 8950
	GO:0008166	viral replication	PMID:24086464	ECO:0000315			P		Figure 3B.Interaction of Hsp70 with deletion mutants of NS5 protein. Hsp70-Myc and a series of deletion mutants of Flag-NS5 were co-transfected in 293T cells, precipitated with mouse anti-Flag MAb and then subjected to Western blotting.	complete CACAO 8951
	GO:0008166	viral replication	PMID:24086464	ECO:0000315			P		Figure 3C.The purified proteins of GST-NS5 (406-905) and His-Hsp70 were analyzed by SDS-PAGE.	complete CACAO 8952
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 3D.The direct interaction between GST-NS5 RdRp domain and His-Hsp70 was analyzed by GST pull-down assay and Western blotting.	complete CACAO 8953
	GO:0008166		PMID:24086464	ECO:0000314					Figure 4A. Interaction of Hsp70 with components of the JEV replicase complex. Interaction of Hsp70 with JEV NS3. 293T cells were co-transfected with Myc-tagged Hsp70 plasmid and Flag-tagged NS3 plasmid or vector. Cell lysates were harvested 36 h after transfection for co-IP experiments with anti-Flag antibody. The precipitates were then analyzed by Western blotting with anti-Flag or anti-Myc antibody.	complete CACAO 8954
	GO:0008166	viral replication	PMID:24086464	ECO:0000314			P		Figure 4 (B) Association of Hsp70 with NS3 and NS5 proteins and viral RNA in JEV-infected cells. 293T cells were transfected with Hsp70-Myc expressing plasmid or vector before being mock-infected or infected with JEV at an MOI of 1.0. Cell lysates were harvested at 36 h p.i. and subjected to immunoprecipitation with antibody against Myc, followed by Western blotting for the detection of NS3 and NS5. The RNA were extracted from the precipitates and then subjected to RT-PCR for detecting viral RNA. RNA extracted from cells infected and uninfected with JEV was used as positive control (PC) and negative control (NC) respectively.	complete CACAO 8955
involved_in	GO:0001172	transcription, RNA-templated	GO_REF:0000108	ECO:0000366	evidence based on logical inference from automatic annotation used in automatic assertion	GO:0003968	P		Seeded From UniProt	complete
enables	GO:0003968	RNA-directed 5'-3' RNA polymerase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000208	F		Seeded From UniProt	complete
enables	GO:0005524	ATP binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000208	F		Seeded From UniProt	complete
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