PSEAE:LASR

Species (Taxon ID)	Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228). (208964)
Gene Name(s)	lasR
Protein Name(s)	Transcriptional activator protein LasR
External Links
UniProt	P25084
EMBL	M59425 D30813 D30812 AE004091
PIR	A43660 S53692 S53693
RefSeq	NP_250121.1
PDB	2UV0 3IX3 3IX4 3IX8 3JPU 4NG2
PDBsum	2UV0 3IX3 3IX4 3IX8 3JPU 4NG2
ProteinModelPortal	P25084
SMR	P25084
STRING	208964.PA1430
BindingDB	P25084
ChEMBL	CHEMBL1075207
DNASU	881789
EnsemblBacteria	AAG04819
GeneID	881789
KEGG	pae:PA1430
PATRIC	19837188
PseudoCAP	PA1430
eggNOG	COG2771
HOGENOM	HOG000111053
InParanoid	P25084
KO	K18304
OMA	HAWHSIT
OrthoDB	EOG69D3BB
PhylomeDB	P25084
EvolutionaryTrace	P25084
Proteomes	UP000002438
GO	GO:0003677 GO:0003700 GO:0009405 GO:0051544 GO:0045893 GO:0009372 GO:0060310 GO:0010468 GO:0006355 GO:0006351
Gene3D	1.10.10.10 3.30.450.80
InterPro	IPR016032 IPR005143 IPR000792 IPR011991
Pfam	PF03472 PF00196
PRINTS	PR00038
SMART	SM00421
SUPFAM	SSF46894 SSF75516
PROSITE	PS00622 PS50043

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Notes	Status
	GO:0045893	positive regulation of transcription, DNA-dependent	PMID:1902216^[1]	ECO:0000315			P	Figure 7 shows that when cells contain a plasmid with a functional LasR and LasB, the LasR induces transcription of LasB transcripts. When there is a plasmid with a deletion mutation in LasR and still contains LasB, there is no transcription of LasB. Thus, showing that LasR positively regulates transcription.	complete
	GO:0009372	quorum sensing	PMID:9150205^[2]	ECO:0000314			P	Figure 3a shows almost a two-fold increase in lasR expression under the presence of autoinducers, PAI-1 and PAI-2, showing that lasR is involved in quorum sensing in P. aeruginosa	complete
	GO:0010468	regulation of gene expression	PMID:9294432^[3]	ECO:0000314			P	Table 3 shows that lasB9-lacZ expression in PAI-1 (50 nM) in the presence of LasR was greatly amplified.	complete CACAO 5028
	GO:0010468	transcription, DNA-dependent	PMID:PMC179088	ECO:0000316		PMID:PMC179088	P	Figure 1	complete CACAO 5068
	GO:0005667	transcription factor complex	PMID:PMC179088	ECO:0000316		PMID:PMC179088	C	From figures 2,3, and 4. The LasR–PAI-1 complex is a global regulator of P. aeruginosa virulence.	complete CACAO 5072
	GO:0045893	positive regulation of transcription, DNA-dependent	PMID:9150205^[2]	ECO:0000314			P	Figure 4. In the absence of LasR or PAI-1, rhlR expression remained at background level, but in the presence of both LasR and PAI-1, rhlR expression increased approximately threefold, indicating that rhlR was directly controlled by LasR and PAI-1.	complete CACAO 5863
	GO:0003700	sequence-specific DNA binding transcription factor activity	PMID:23066081^[4]	ECO:0000315			F	Figure 1.section 3 shows the mutant strain in the Lux R protein and what happens in the presence of adenosine.	complete CACAO 8650
NOT	GO:0016563	transcription activator activity	PMID:24157832^[5]	ECO:0000314			F	Figure 8. AmpR positively regulates critical QS regulators of the Las system, Rhl system and PQS. Expression of the genes was determined by qPCR.	complete CACAO 8937
	GO:0006979	response to oxidative stress	PMID:27053110^[6]	ECO:0000315			P	Figure 3.A. Proteins shifted DNA in the absence of oxidant.	complete CACAO 11702
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	PMID:9150205^[2]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0010468	regulation of gene expression	PMID:9294432^[3]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0045893	positive regulation of transcription, DNA-templated	PMID:12076794^[7]	ECO:0005657	primer extension assay evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:18045385^[8]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_002516.2	F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:15505212^[9]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_002516.2	F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:18045385^[8]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0043565	sequence-specific DNA binding	PMID:15505212^[9]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:19682264^[10]	ECO:0006007	chromatin immunoprecipitation-chip evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:8576049^[11]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:20233934^[12]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:17449616^[13]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:16735731^[14]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:11544214^[15]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:18045385^[8]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_002516.2	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:15505212^[9]	ECO:0005631	DNAse footprinting evidence used in manual assertion	RefSeq:NC_002516.2	C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:19682264^[10]	ECO:0005620	chromatin immunoprecipitation-PCR evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:20233934^[12]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:19682264^[10]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:18045385^[8]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:17449616^[13]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
part_of	GO:0032993	protein-DNA complex	PMID:15505212^[9]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		C	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:19682264^[10]	ECO:0006007	chromatin immunoprecipitation-chip evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:8576049^[11]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:20233934^[12]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:17449616^[13]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:16735731^[14]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:11544214^[15]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:19682264^[10]	ECO:0005620	chromatin immunoprecipitation-PCR evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:20233934^[12]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:19682264^[10]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0001216	DNA-binding transcription activator activity	PMID:17449616^[13]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:19682264^[10]	ECO:0006007	chromatin immunoprecipitation-chip evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:8576049^[11]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:20233934^[12]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:17449616^[13]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:16735731^[14]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:11544214^[15]	ECO:0005667	site-directed mutagenesis evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:19682264^[10]	ECO:0005620	chromatin immunoprecipitation-PCR evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:20233934^[12]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:19682264^[10]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
enables	GO:0000976	transcription regulatory region sequence-specific DNA binding	PMID:17449616^[13]	ECO:0001807	electrophoretic mobility shift assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0051544	positive regulation of elastin biosynthetic process	PMID:1902216^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0009405	pathogenesis	PMID:8454322^[16]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	PMID:1902216^[1]	ECO:0000314	direct assay evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0003700	DNA-binding transcription factor activity	PMID:1902216^[1]	ECO:0000314	direct assay evidence used in manual assertion		F	Seeded From UniProt	complete
involved_in	GO:0060310	regulation of elastin catabolic process	PMID:1902216^[1]	ECO:0000315	mutant phenotype evidence used in manual assertion		P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR016032	F	Seeded From UniProt	complete
involved_in	GO:0006355	regulation of transcription, DNA-templated	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR000792 InterPro:IPR016032	P	Seeded From UniProt	complete
involved_in	GO:0009372	quorum sensing	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0673	P	Seeded From UniProt	complete
enables	GO:0003677	DNA binding	GO_REF:0000037	ECO:0000322	imported manually asserted information used in automatic assertion	UniProtKB-KW:KW-0238	F	Seeded From UniProt	complete
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Notes

References

See Help:References for how to manage references in GONUTS.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 Gambello, MJ & Iglewski, BH (1991) Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J. Bacteriol. 173 3000-9 PubMed GONUTS page
↑ ^2.0 ^2.1 ^2.2 Pesci, EC et al. (1997) Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179 3127-32 PubMed GONUTS page
↑ ^3.0 ^3.1 Pearson, JP et al. (1997) Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179 5756-67 PubMed GONUTS page
↑ Dandekar, AA et al. (2012) Bacterial quorum sensing and metabolic incentives to cooperate. Science 338 264-6 PubMed GONUTS page
↑ Balasubramanian, D et al. (2014) Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response. Nucleic Acids Res. 42 979-98 PubMed GONUTS page
↑ Kafle, P et al. (2016) Molecular Insights into the Impact of Oxidative Stress on Quorum Sensing Regulator Protein LasR. J. Biol. Chem. PubMed GONUTS page
↑ de Kievit, TR et al. (2002) Role of the Pseudomonas aeruginosa las and rhl quorum-sensing systems in rhlI regulation. FEMS Microbiol. Lett. 212 101-6 PubMed GONUTS page
↑ ^8.0 ^8.1 ^8.2 ^8.3 Rampioni, G et al. (2007) RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa. Mol. Microbiol. 66 1557-65 PubMed GONUTS page
↑ ^9.0 ^9.1 ^9.2 ^9.3 Schuster, M et al. (2004) Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Proc. Natl. Acad. Sci. U.S.A. 101 15833-9 PubMed GONUTS page
↑ ^10.0 ^10.1 ^10.2 ^10.3 ^10.4 ^10.5 ^10.6 ^10.7 ^10.8 Gilbert, KB et al. (2009) Global position analysis of the Pseudomonas aeruginosa quorum-sensing transcription factor LasR. Mol. Microbiol. 73 1072-85 PubMed GONUTS page
↑ ^11.0 ^11.1 ^11.2 Rust, L et al. (1996) Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region. J. Bacteriol. 178 1134-40 PubMed GONUTS page
↑ ^12.0 ^12.1 ^12.2 ^12.3 ^12.4 ^12.5 Thaden, JT et al. (2010) Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. J. Bacteriol. 192 2557-68 PubMed GONUTS page
↑ ^13.0 ^13.1 ^13.2 ^13.3 ^13.4 ^13.5 Li, LL et al. (2007) Regulation of the Pseudomonas aeruginosa quorum-sensing regulator VqsR. J. Bacteriol. 189 4367-74 PubMed GONUTS page
↑ ^14.0 ^14.1 ^14.2 Xiao, G et al. (2006) Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry. Microbiology (Reading, Engl.) 152 1679-86 PubMed GONUTS page
↑ ^15.0 ^15.1 ^15.2 Whiteley, M & Greenberg, EP (2001) Promoter specificity elements in Pseudomonas aeruginosa quorum-sensing-controlled genes. J. Bacteriol. 183 5529-34 PubMed GONUTS page
↑ Gambello, MJ et al. (1993) LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression. Infect. Immun. 61 1180-4 PubMed GONUTS page

[PMID:1902216-1] 1.0 ^1.1 ^1.2 ^1.3 ^1.4 Gambello, MJ & Iglewski, BH (1991) Cloning and characterization of the Pseudomonas aeruginosa lasR gene, a transcriptional activator of elastase expression. J. Bacteriol. 173 3000-9 PubMed GONUTS page

[PMID:9150205-2] 2.0 ^2.1 ^2.2 Pesci, EC et al. (1997) Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179 3127-32 PubMed GONUTS page

[PMID:9294432-3] 3.0 ^3.1 Pearson, JP et al. (1997) Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179 5756-67 PubMed GONUTS page

[PMID:23066081-4] Dandekar, AA et al. (2012) Bacterial quorum sensing and metabolic incentives to cooperate. Science 338 264-6 PubMed GONUTS page

[PMID:24157832-5] Balasubramanian, D et al. (2014) Deep sequencing analyses expands the Pseudomonas aeruginosa AmpR regulon to include small RNA-mediated regulation of iron acquisition, heat shock and oxidative stress response. Nucleic Acids Res. 42 979-98 PubMed GONUTS page

[PMID:27053110-6] Kafle, P et al. (2016) Molecular Insights into the Impact of Oxidative Stress on Quorum Sensing Regulator Protein LasR. J. Biol. Chem. PubMed GONUTS page

[PMID:12076794-7] Kievit, TR et al. (2002) Role of the Pseudomonas aeruginosa las and rhl quorum-sensing systems in rhlI regulation. FEMS Microbiol. Lett. 212 101-6 PubMed GONUTS page

[PMID:18045385-8] 8.0 ^8.1 ^8.2 ^8.3 Rampioni, G et al. (2007) RsaL provides quorum sensing homeostasis and functions as a global regulator of gene expression in Pseudomonas aeruginosa. Mol. Microbiol. 66 1557-65 PubMed GONUTS page

[PMID:15505212-9] 9.0 ^9.1 ^9.2 ^9.3 Schuster, M et al. (2004) Promoter specificity in Pseudomonas aeruginosa quorum sensing revealed by DNA binding of purified LasR. Proc. Natl. Acad. Sci. U.S.A. 101 15833-9 PubMed GONUTS page

[PMID:19682264-10] 10.0 ^10.1 ^10.2 ^10.3 ^10.4 ^10.5 ^10.6 ^10.7 ^10.8 Gilbert, KB et al. (2009) Global position analysis of the Pseudomonas aeruginosa quorum-sensing transcription factor LasR. Mol. Microbiol. 73 1072-85 PubMed GONUTS page

[PMID:8576049-11] 11.0 ^11.1 ^11.2 Rust, L et al. (1996) Analysis of the Pseudomonas aeruginosa elastase (lasB) regulatory region. J. Bacteriol. 178 1134-40 PubMed GONUTS page

[PMID:20233934-12] 12.0 ^12.1 ^12.2 ^12.3 ^12.4 ^12.5 Thaden, JT et al. (2010) Quorum-sensing regulation of a copper toxicity system in Pseudomonas aeruginosa. J. Bacteriol. 192 2557-68 PubMed GONUTS page

[PMID:17449616-13] 13.0 ^13.1 ^13.2 ^13.3 ^13.4 ^13.5 Li, LL et al. (2007) Regulation of the Pseudomonas aeruginosa quorum-sensing regulator VqsR. J. Bacteriol. 189 4367-74 PubMed GONUTS page

[PMID:16735731-14] 14.0 ^14.1 ^14.2 Xiao, G et al. (2006) Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry. Microbiology (Reading, Engl.) 152 1679-86 PubMed GONUTS page

[PMID:11544214-15] 15.0 ^15.1 ^15.2 Whiteley, M & Greenberg, EP (2001) Promoter specificity elements in Pseudomonas aeruginosa quorum-sensing-controlled genes. J. Bacteriol. 183 5529-34 PubMed GONUTS page

[PMID:8454322-16] Gambello, MJ et al. (1993) LasR of Pseudomonas aeruginosa is a transcriptional activator of the alkaline protease gene (apr) and an enhancer of exotoxin A expression. Infect. Immun. 61 1180-4 PubMed GONUTS page

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PSEAE:LASR

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