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PMID:19682264

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Citation

Gilbert, KB, Kim, TH, Gupta, R, Greenberg, EP and Schuster, M (2009) Global position analysis of the Pseudomonas aeruginosa quorum-sensing transcription factor LasR. Mol. Microbiol. 73:1072-85

Abstract

In Pseudomonas aeruginosa quorum sensing (QS), the transcriptional regulator LasR controls the expression of more than 300 genes. Several of these genes are activated indirectly via a second, subordinate QS regulator, RhlR. Conserved sequence elements upstream of individual other genes have been shown to bind LasR in vitro. To comprehensively identify all regions that are bound by LasR in vivo, we employed chromatin immunoprecipitation in conjunction with microarray analysis. We identified 35 putative promoter regions that direct the expression of up to 74 genes. In vitro DNA binding studies allowed us to distinguish between cooperative and non-cooperative LasR binding sites, and allowed us to build consensus sequences according to the mode of binding. Five promoter regions were not previously recognized as QS-controlled. Two of the associated transcript units encode proteins involved in the cold-shock response and in Psl exopolysaccharide synthesis respectively. The LasR regulon includes seven genes encoding transcriptional regulators, while secreted factors and secretion machinery are the most over-represented functional categories overall. This supports the notion that the core function of LasR is to co-ordinate the production of extracellular factors, although many of its effects on global gene expression are likely mediated indirectly by regulatory genes under its control.

Links

PubMed PMC2759405 Online version:10.1111/j.1365-2958.2009.06832.x

Keywords

Bacterial Proteins/metabolism; Binding Sites; Chromatin Immunoprecipitation; DNA, Bacterial/metabolism; Gene Expression Regulation, Bacterial; Microarray Analysis; Promoter Regions, Genetic; Protein Binding; Pseudomonas aeruginosa/physiology; Quorum Sensing; Regulon; Trans-Activators/metabolism

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

PSEAE:LASR

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0005620: chromatin immunoprecipitation-PCR evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASR

part_of

GO:0032993: protein-DNA complex

ECO:0006007: chromatin immunoprecipitation-chip evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:LASR

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0006007: chromatin immunoprecipitation-chip evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASR

part_of

GO:0032993: protein-DNA complex

ECO:0001807: electrophoretic mobility shift assay evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:LASR

part_of

GO:0032993: protein-DNA complex

ECO:0005620: chromatin immunoprecipitation-PCR evidence used in manual assertion

C

Seeded From UniProt

complete

PSEAE:LASR

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0006007: chromatin immunoprecipitation-chip evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASR

enables

GO:0001216: DNA-binding transcription activator activity

ECO:0001807: electrophoretic mobility shift assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASR

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0001807: electrophoretic mobility shift assay evidence used in manual assertion

F

Seeded From UniProt

complete

PSEAE:LASR

enables

GO:0000976: transcription regulatory region sequence-specific DNA binding

ECO:0005620: chromatin immunoprecipitation-PCR evidence used in manual assertion

F

Seeded From UniProt

complete

Notes

See also

References

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