User:Jolive15

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	PSEST:NOSZ	2011-10-18 17:44:29 CDT	GO:0015677 copper ion import (P)	PMID:2170125	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 2: Copper content in cells of mutant phenotypes drops dramatically indicating cells cannot import copper.	challenge
acceptable	ECOLI:NARI	2011-10-22 21:57:22 CDT	GO:0009325 nitrate reductase complex (C)	PMID:3053688	ECO:0000314 direct assay evidence used in manual assertion	Figure 4. Column 2 (0 min); Column 3 (2min) Column 4 (10min). As length of purification process increases, expression decreases - less expression in each consecutive column after column 2. Evidence that narJ is lost during lengthy purification process.	challenge
acceptable	EMENI:NIRA	2011-10-22 22:02:13 CDT	GO:0003700 sequence-specific DNA binding transcription factor activity (F)	PMID:1922075	ECO:0000314 direct assay evidence used in manual assertion	Figure 6,7. These figures show that the NIRA protein binds to the cis-acting sites of niaD and niiA.	challenge
acceptable	ECOLI:NAPA	2011-10-23 13:53:46 CDT	GO:0030288 outer membrane-bounded periplasmic space (C)	PMID:10234835	ECO:0000314 direct assay evidence used in manual assertion	Figure 1. Periplasmic proteins separated and stained for nitrate reductase activity. Banding seen is due to the reduction of nitrite formed as the product of NapA activity by periplasmic nitrite reductase [20].	challenge
acceptable	ECOLI:NAPA	2011-10-23 14:15:29 CDT	GO:0008940 nitrate reductase activity (F)	PMID:10234835	ECO:0000314 direct assay evidence used in manual assertion	Nap activity was detected, but associated with a band of decreased electrophoretic mobility which lacks c-type cytochrome, implying that NapA is active with the artificial electron donor, methyl viologen, in the absence of NapB (Fig. 1, track 2).	challenge
acceptable	PSEST:NORB	2011-10-23 17:32:32 CDT	GO:0016966 nitric oxide reductase activity (F)	PMID:7508388	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 8. Deletion-replacement mutagenesis of the gene norB resulted in the loss of NO reduction of P stutzeri strain MK321.	challenge
acceptable	RHOSH:O88111	2011-11-04 19:48:02 CDT	GO:0071941 nitrogen cycle metabolic process (P)	PMID:10227138	ECO:0000315 mutant phenotype evidence used in manual assertion	Table 3. Genes napKEFDABC were determined to be involved in denitrification in an experiment that measured gases released by four strains: wild-type, nap-bearing, napD-deficient mutant and non-nap bearing mutant that conjugated with another bacterium containing napKEFDABC. Wild-type strain produced gases which showed denitrification activity. napD deficient strain as well as non-nap bearing strain produced no gases and therefore no denitrification activity. Nap-deficient mutant that conjugated with nap-bearing bacterium regained denitrification activities.	challenge

acceptable:7
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Jolive15

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Helioslaw, Team Jimmy Johns	BACSU:ABRB	GO:0043937 - regulation of sporulation (P)	PMID:15057450	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 2
acceptable	Dhaase1, Team Step 1 Nitrification	NITEU:AMOA	GO:0004497 - monooxygenase activity (F)	PMID:16349014	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 2
acceptable	Jolive15, Team Denitrifiers	ECOLI:NAPA	GO:0030288 - outer membrane-bounded periplasmic space (C)	PMID:10234835	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 3

0 annotations fixed by Jolive15