GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
|Status||Page||Date/Time||GO Term (Aspect)||Reference||Evidence||Notes||Links|
|PSEST:NOSZ||2011-10-18 17:44:29 CDT||GO:0015677 copper ion import (P)||PMID:2170125||ECO:0000315 mutant phenotype evidence used in manual assertion|
Table 2: Copper content in cells of mutant phenotypes drops dramatically indicating cells cannot import copper.
|ECOLI:NARI||2011-10-22 21:57:22 CDT||GO:0009325 nitrate reductase complex (C)||PMID:3053688||ECO:0000314 direct assay evidence used in manual assertion|
Figure 4. Column 2 (0 min); Column 3 (2min) Column 4 (10min). As length of purification process increases, expression decreases - less expression in each consecutive column after column 2. Evidence that narJ is lost during lengthy purification process.
|EMENI:NIRA||2011-10-22 22:02:13 CDT||GO:0003700 sequence-specific DNA binding transcription factor activity (F)||PMID:1922075||ECO:0000314 direct assay evidence used in manual assertion|
Figure 6,7. These figures show that the NIRA protein binds to the cis-acting sites of niaD and niiA.
|ECOLI:NAPA||2011-10-23 13:53:46 CDT||GO:0030288 outer membrane-bounded periplasmic space (C)||PMID:10234835||ECO:0000314 direct assay evidence used in manual assertion|
Figure 1. Periplasmic proteins separated and stained for nitrate reductase activity. Banding seen is due to the reduction of nitrite formed as the product of NapA activity by periplasmic nitrite reductase .
|ECOLI:NAPA||2011-10-23 14:15:29 CDT||GO:0008940 nitrate reductase activity (F)||PMID:10234835||ECO:0000314 direct assay evidence used in manual assertion|
Nap activity was detected, but associated with a band of decreased electrophoretic mobility which lacks c-type cytochrome, implying that NapA is active with the artificial electron donor, methyl viologen, in the absence of NapB (Fig. 1, track 2).
|PSEST:NORB||2011-10-23 17:32:32 CDT||GO:0016966 nitric oxide reductase activity (F)||PMID:7508388||ECO:0000315 mutant phenotype evidence used in manual assertion|
Figure 8. Deletion-replacement mutagenesis of the gene norB resulted in the loss of NO reduction of P stutzeri strain MK321.
|RHOSH:O88111||2011-11-04 19:48:02 CDT||GO:0071941 nitrogen cycle metabolic process (P)||PMID:10227138||ECO:0000315 mutant phenotype evidence used in manual assertion|
Table 3. Genes napKEFDABC were determined to be involved in denitrification in an experiment that measured gases released by four strains: wild-type, nap-bearing, napD-deficient mutant and non-nap bearing mutant that conjugated with another bacterium containing napKEFDABC. Wild-type strain produced gases which showed denitrification activity. napD deficient strain as well as non-nap bearing strain produced no gases and therefore no denitrification activity. Nap-deficient mutant that conjugated with nap-bearing bacterium regained denitrification activities.
Annotations challenged by Jolive15
|Status||Author,Group||Page||GO Term (Aspect)||Reference||Evidence||Links||Page history|
Team Jimmy Johns
|BACSU:ABRB||GO:0043937 - regulation of sporulation (P)||PMID:15057450||ECO:0000316 genetic interaction evidence used in manual assertion||challenge||C: 2|
Team Step 1 Nitrification
|NITEU:AMOA||GO:0004497 - monooxygenase activity (F)||PMID:16349014||ECO:0000314 direct assay evidence used in manual assertion||challenge||C: 2|
|ECOLI:NAPA||GO:0030288 - outer membrane-bounded periplasmic space (C)||PMID:10234835||ECO:0000314 direct assay evidence used in manual assertion||challenge||C: 3|
0 annotations fixed by Jolive15