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User:Helioslaw

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My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableBACSU:PTHP2011-09-23 11:22:59 CDTGO:0043610 regulation of carbohydrate utilization (P)PMID:8195089ECO:0000315 mutant phenotype evidence used in manual assertion

Table 2 shows enzyme activity for gluconate kinase not repressed in mutant

challenge
acceptableBACSU:CCPN2011-09-23 12:49:51 CDTGO:0045013 carbon catabolite repression of transcription (P)PMID:15720552ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that mutant CcpN more fully expressed gabB leading to the conclusion that CcpN acts as a negative regulator of gapB transcription

challenge
acceptableBACSU:YYCF2011-09-24 11:48:40 CDTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:10878122ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows direct relationship between Yycf and the expression of P1 promoter of ftszAZ operon

challenge
acceptableBACSU:ESTA2011-09-24 20:36:13 CDTGO:0004806 triglyceride lipase activity (F)PMID:8396026ECO:0000314 direct assay evidence used in manual assertion

Table 2 shows esterase activity of estA lipase

challenge
acceptableBACSU:RECU2011-09-25 17:55:03 CDTGO:0006281 DNA repair (P)PMID:9642195ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1a shows inability of mutant recU organism to survive in the presence of MMS while wild rec+ type cells are able to survive

challenge
acceptableBACSU:DESK2011-10-04 19:39:02 CDTGO:0004721 phosphoprotein phosphatase activity (F)PMID:15090506ECO:0000314 direct assay evidence used in manual assertion

Fig. 3 A shows DesKC phosphatase activity

challenge
acceptableBACSU:FLIY2011-10-07 11:29:07 CDTGO:0004721 phosphoprotein phosphatase activity (F)PMID:12920116ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows Fliy activity in dephosphorylation of Chey-P

challenge
acceptableBACSU:RSBW2011-10-07 11:57:34 CDTGO:0016989 sigma factor antagonist activity (F)PMID:8468294ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 4 shows a large increase in the sigma b protein in rsbW mutants

challenge
acceptableBACSU:DAGK2011-10-08 10:17:51 CDTGO:0004143 diacylglycerol kinase activity (F)PMID:17535816ECO:0000314 direct assay evidence used in manual assertion

Figure 4 B,C show Dgkb kinase activity

challenge
acceptableBACSU:SP2E2011-10-18 20:04:37 CDTGO:0042601 endospore-forming forespore (C)PMID:18077456ECO:0000314 direct assay evidence used in manual assertion

Figure 2. Shows SpollE is located throughout forespore membrane after polar division but concentrated at septal membrane during polar division

challenge
acceptableBACSU:SP2Q2011-10-18 20:14:19 CDTGO:0042601 endospore-forming forespore (C)PMID:18077456ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows location of SpollQ in relation to SpollE by showing SpollQ mutant failing to relocalizing SpollE to septal membrane

challenge
acceptableBACSU:YABQ2011-10-18 20:36:11 CDTGO:0042601 endospore-forming forespore (C)PMID:11283287ECO:0000314 direct assay evidence used in manual assertion

Figure 5 a,b shows location of yabQ in spore membrane

challenge
acceptableBACSU:SP0E2011-10-18 21:03:54 CDTGO:0043937 regulation of sporulation (P)PMID:15057450ECO:0000316 genetic interaction evidence used in manual assertion

Table 2 shows that mutant SpoE causes an increase in sporulation

challenge
acceptableBACSU:ABRB2011-10-21 11:11:28 CDTGO:0043937 regulation of sporulation (P)PMID:15057450ECO:0000316 genetic interaction evidence used in manual assertion

Table 2

challenge
acceptableBACSU:KINA2011-10-22 10:58:19 CDTGO:0043938 positive regulation of sporulation (P)PMID:15057450ECO:0000316 genetic interaction evidence used in manual assertion

Figure 2 shows kinase mutation effects on abrB expression, abrB is a negative regulator of sporulation

challenge
acceptableBACSU:SP0J2011-11-03 13:34:10 CDTGO:0007059 chromosome segregation (P)PMID:8071208ECO:0000315 mutant phenotype evidence used in manual assertion

Table 5 shows accumulation in anucleate cells

challenge
acceptableBACSU:SOJ2011-11-03 13:44:59 CDTGO:0042174 negative regulation of sporulation resulting in formation of a cellular spore (P)PMID:8071208ECO:0000316 genetic interaction evidence used in manual assertion

Table 3

challenge
acceptableBACSU:SP0J2011-11-03 13:50:49 CDTGO:0045881 positive regulation of sporulation resulting in formation of a cellular spore (P)PMID:8071208ECO:0000316 genetic interaction evidence used in manual assertion

Figure 2 shows that spoJ, in concert with soj regulates the levels of downstream sporulation genes.

Tables 3 & 4 establish that spoJ is dependent on soj for positive regulation of sporulation.

challenge
acceptableBACSU:YABP2011-11-04 10:45:57 CDTGO:0042601 endospore-forming forespore (C)PMID:15231775ECO:0000314 direct assay evidence used in manual assertion

Figure 1 A shows location of yabP in the forespore membrane

challenge
acceptableBACSU:YHED2011-11-04 10:52:02 CDTGO:0042601 endospore-forming forespore (C)PMID:15231775ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows location of Yhe-D in forespore membrane

challenge
acceptableBACSU:YUTH2011-11-04 10:54:47 CDTGO:0042601 endospore-forming forespore (C)PMID:15231775ECO:0000314 direct assay evidence used in manual assertion

Figure 6. Membrane.

challenge
acceptableBACSU:SINR2011-11-15 19:46:35 CSTGO:0010629 negative regulation of gene expression (P)PMID:15661000ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1

challenge
acceptableBACSU:HPR2011-11-15 20:01:52 CSTGO:0006355 regulation of transcription, DNA-dependent (P)PMID:10383984ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2

challenge
updatedbyinstructorBACSU:APBC2011-11-15 20:15:16 CSTGO:0045892 regulation of transcription, DNA-dependent (P)PMID:15126467ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 3 and Fig 5

challenge
acceptableBACSU:YWCE2011-11-18 11:23:55 CSTGO:0009847 spore germination (P)PMID:16159778ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7

challenge

acceptable:24
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Helioslaw

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
acceptableSakurascorpion,
Team Beta-Blockers
HCVJ6:POLGGO:0016032 - viral process (P)PMID:17634240ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableTaustin,
Team Big Mama 50000
PSEAE:Q9I2Q1GO:0070011 - peptidase activity, acting on L-amino acid peptides (F)PMID:21776080ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
acceptableMkline,
Team Cheesesteak
HUMAN:LATGO:0045860 - positive regulation of protein kinase activity (P)PMID:14624253ECO:0000270 expression pattern evidence used in manual assertionchallengeC: 1
acceptableMkline,
Team Cheesesteak
HUMAN:LCP2GO:0045860 - positive regulation of protein kinase activity (P)PMID:14624253ECO:0000270 expression pattern evidence used in manual assertionchallengeC: 1
acceptableElleon,
Team Beta-Blockers
XENLA:WEE1BGO:0045786 - negative regulation of cell cycle (P)PMID:17961226ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptableSakurascorpion,
Team Beta-Blockers
HUMAN:PGAM5GO:0004722 - protein serine/threonine phosphatase activity (F)PMID:19590015ECO:0000314 direct assay evidence used in manual assertionchallengeC: 1
acceptableHelioslaw,
Team Jimmy Johns
BACSU:ABRBGO:0043937 - regulation of sporulation (P)PMID:15057450ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 2
acceptableLrodgers,
Team KremlinOnTheCrum
MAGGR:Q875L7GO:0006111 - regulation of gluconeogenesis (P)PMID:12514128ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
acceptableEmlarkin03,
Team Angry Squirrels
ECOLI:PTGCBGO:0016021 - integral component of membrane (C)PMID:3129430ECO:0000255 match to sequence model evidence used in manual assertionchallengeC: 2
acceptableHessa,
Team Biology Bandits
MOUSE:AHI1GO:0046549 - retinal cone cell development (P)PMID:20081859ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1
acceptableHessa,
Team Biology Bandits
MOUSE:AHI1GO:0050708 - regulation of protein secretion (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 2
acceptableHessa,
Team Biology Bandits
MOUSE:AHI1GO:0010976 - positive regulation of neuron projection development (P)PMID:18636121ECO:0000315 mutant phenotype evidence used in manual assertionchallengeC: 1

0 annotations fixed by Helioslaw