User:Indra.Bhattarai

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	STAAU:A0A0E1X950	2016-03-05 18:34:28 CST	GO:0016020 membrane (C)	PMID:22492855	ECO:0000315 mutant phenotype evidence used in manual assertion	figure A. shows Vancomycin minimum inhibitory concentrations (MICs) were determined by Etest for A5937, A5937Δstp1, the complemented strain A5937Δstp1-C, and the vancomycin nonsusceptible isolate A5940. Values in parentheses represent MICs based on broth dilution.Figure B. shows Determination of vancomycin heteroresistance was performed using population analysis profiling. The stp1 deletion mutant had subpopulations that grew in the nonsusceptible range (>2 µg/mL); this was not seen in the parent strain (A5937) but was more pronounced in the clinical vancomycin-intermediate S. aureus daughter strain (A5940). CFU, colony-forming unit. Figure C. shows transmission electron microscopy was performed to determine cell wall thickness. Three representative cells from each strain are shown, and values represent mean cell wall thickness (95% confidence interval).	challenge
unacceptable	STAAU:A0A0M1TZU6	2016-03-08 17:35:17 CST	GO:0008658 penicillin binding (Antibiotic susceptibility test was conducted using antibiotics according to the guidelines of Clinical and Laboratory Standards Institute. Also,Phenotypic biofilm formation was performed with microtiter plate assay.)	PMID:21800213	IDA: Inferred from Direct Assay		challenge
unacceptable	STAAU:O54286	2016-04-19 03:47:29 CDT	GO:0046677 response to antibiotic (P)	PMID:26833147	ECO:0000315 mutant phenotype evidence used in manual assertion	Seeded from Uniport	challenge

acceptable:0
unacceptable:3
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Annotations challenged by Indra.Bhattarai

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	Nathan.Marsh, Team Grapes of Staph	ALIFS:LUXR	GO:1905087 - positive regulation of bioluminescence (P)	PMID:10913092	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1
unacceptable	Saywala.Massah, Team I'm bringing sexy bacterium	MOUSE:Q3UEK1	GO:0044130 - obsolete negative regulation of growth of symbiont in host (P)	PMID:27058056	ECO:0000316 genetic interaction evidence used in manual assertion	challenge	C: 2

0 annotations fixed by Indra.Bhattarai

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