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User:Nathan.Marsh

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CACAO Spring 2016

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableALIFS:LUXI2016-03-07 17:52:42 CSTGO:1905087 positive regulation of bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that when autoinducers are introduced to the LuxI mutant then the bacteria will bioluminesce. Fig 2 signifies that when the luxI is not present the bioluminescence is significantly decreased in the colonized juvenile squid.

challenge
acceptableALIFS:LUXR2016-03-07 17:56:11 CSTGO:1905087 positive regulation of bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 displays results of autoinducing molecules that can trigger the LuxR mutant into bioluminescing. Fig 2 signifies that when the luxR is not present the bioluminescence is significantly decreased in the colonized juvenile squids.

challenge
acceptableALIFS:LUXA2016-04-11 05:15:25 CDTGO:0008218 bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that the LuxA gene is necessary for bioluminescence (not a regulator). Figure 2 also shows that biolumiscence is low in the LuxA mutants of the colonized juvenile squids in the experiment.

challenge
updatedbyinstructorALIFS:LUXI2016-04-11 07:24:56 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows the different cytoplasmic volumes of the mutants and uninfected vs the wild type.

challenge
updatedbyinstructorALIFS:LUXR2016-04-11 07:31:57 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

The table in Figure 5 shows the decreased levels of cytoplasm in the LuxR mutant vs the uninfected squids and wild type infected squids.

challenge
updatedbyinstructorALIFS:LUXA2016-04-11 07:47:27 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows TEMs of the narrow columnar cells of the LuxA mutant infected squids vs the enlarged columnar cells of the wild type infected squids. The graph also depicts the decreased cytoplasmic volume between the two.

challenge

acceptable:3
unacceptable:0
requires_changes:0
flagged:0

Annotations challenged by Nathan.Marsh

StatusAuthor,GroupPageGO Term (Aspect)ReferenceEvidenceLinksPage history
updatedbyinstructorSydney.B.Bork,
Team Alpha
LISMO:CSPAGO:0019836 - hemolysis by symbiont of host erythrocytes (P)PMID:23952475ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 4
updatedbyinstructorNicole.Lapage,
Team Alpha
LISIN:CSPBGO:0045893 - positive regulation of DNA-templated transcription (P)PMID:23952475ECO:0000316 genetic interaction evidence used in manual assertionchallengeC: 2
updatedbyinstructorTracy.Pham,
Team Espresso my genes
HUMAN:NLRC4GO:0050713 - negative regulation of interleukin-1 beta secretion (P)PMID:15107016ECO:0000314 direct assay evidence used in manual assertionchallengeC: 3

0 annotations fixed by Nathan.Marsh

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