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Category:Team The Herd

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableSTAAU:O54286Indra.Bhattarai, Team The Herd2016-04-19 03:47:29 CDTGO:0046677 response to antibiotic (P)PMID:26833147ECO:0000315 mutant phenotype evidence used in manual assertion

Seeded from Uniport

challenge
unacceptableSTAA3:A0A0G2Q3K2Dre.E.Steinwehr, Team The Herd2016-04-13 06:36:16 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows the impact of mutations in individual protease genes/operons on biofilm formation in vitro. The relative capacity to form a biofilm was assessed using a microtiter plate assay as previously described (Beenken et al. 2003) using FPR3757, its sarA mutant, and its sarA mutant carrying mutations in the indicated protease genes. Single asterisks indicate significance by comparison to the parent strain. Double asterisks indicate significance by comparison to the sarA mutant. As a control, biofilm formation was also assessed in LAC, its sarA mutant, and derivatives of thesarA mutant unable to produce aureolysin (Saur), unable to produce any extracellular protease (SP), or unable to produce any extracellular protease other than those encoded by the spl operon (SPspl+). Single asterisk indicates statistical significance by comparison to the sarA mutant. Double asterisks indicate significance by comparison to the Saur mutant.

challenge
unacceptableSTAAU:SSPADre.E.Steinwehr, Team The Herd2016-04-13 06:35:20 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows the impact of mutations in individual protease genes/operons on biofilm formation in vitro. The relative capacity to form a biofilm was assessed using a microtiter plate assay as previously described (Beenken et al. 2003) using FPR3757, its sarA mutant, and its sarA mutant carrying mutations in the indicated protease genes. Single asterisks indicate significance by comparison to the parent strain. Double asterisks indicate significance by comparison to the sarA mutant. As a control, biofilm formation was also assessed in LAC, its sarA mutant, and derivatives of thesarA mutant unable to produce aureolysin (Saur), unable to produce any extracellular protease (SP), or unable to produce any extracellular protease other than those encoded by the spl operon (SPspl+). Single asterisk indicates statistical significance by comparison to the sarA mutant. Double asterisks indicate significance by comparison to the Saur mutant.

challenge
unacceptableSTAAU:AUREDre.E.Steinwehr, Team The Herd2016-04-13 06:33:18 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows the impact of mutations in individual protease genes/operons on biofilm formation in vitro. The relative capacity to form a biofilm was assessed using a microtiter plate assay as previously described (Beenken et al. 2003) using FPR3757, its sarA mutant, and its sarA mutant carrying mutations in the indicated protease genes. Single asterisks indicate significance by comparison to the parent strain. Double asterisks indicate significance by comparison to the sarA mutant. As a control, biofilm formation was also assessed in LAC, its sarA mutant, and derivatives of thesarA mutant unable to produce aureolysin (Saur), unable to produce any extracellular protease (SP), or unable to produce any extracellular protease other than those encoded by the spl operon (SPspl+). Single asterisk indicates statistical significance by comparison to the sarA mutant. Double asterisks indicate significance by comparison to the Saur mutant.

challenge
updatedbyinstructorHUMAN:RIN2Dre.E.Steinwehr, Team The Herd2016-04-11 11:39:03 CDTGO:0010595 positive regulation of endothelial cell migration (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
updatedbyinstructorHUMAN:RIN2Dre.E.Steinwehr, Team The Herd2016-04-11 11:39:03 CDTGO:2001214 positive regulation of vasculogenesis (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
unacceptableHUMAN:RIN2Dre.E.Steinwehr, Team The Herd2016-04-11 11:39:03 CDTGO:0050840 extracellular matrix binding (F)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
updatedbyinstructorHUMAN:RRASDre.E.Steinwehr, Team The Herd2016-04-11 11:34:17 CDTGO:0010595 positive regulation of endothelial cell migration (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
updatedbyinstructorHUMAN:RRASDre.E.Steinwehr, Team The Herd2016-04-11 11:34:17 CDTGO:2001214 positive regulation of vasculogenesis (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
updatedbyinstructorHUMAN:RRASDre.E.Steinwehr, Team The Herd2016-04-11 11:34:17 CDTGO:1904906 positive regulation of endothelial cell-matrix adhesion via fibronectin (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
updatedbyinstructorHUMAN:RIN2Dre.E.Steinwehr, Team The Herd2016-04-11 11:23:07 CDTGO:1904906 positive regulation of endothelial cell-matrix adhesion via fibronectin (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
unacceptableHUMAN:RRASDre.E.Steinwehr, Team The Herd2016-04-11 11:18:14 CDTGO:0045785 positive regulation of cell adhesion (P)PMID:22825554ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1. R-Ras and RIN2 promote EC-to-ECM binding, adhesion, migration, and vascular morphogenesis; RIN2 mediates the pro-adhesive effects of R-Ras.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-23 03:33:08 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000316 genetic interaction evidence used in manual assertion

Figure 6 shows the impact of mutating individual protease genes or operons in both the WT and sarA mutant as well as the derivatives of the sarA mutant in terms of biofilm formation.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-23 03:23:50 CDTGO:0006508 proteolysis (P)PMID:25257373ECO:0000316 genetic interaction evidence used in manual assertion

Figure 3 shows the impact of mutating individual protease genes or operons in the WT and sarA mutant as well as the derivatives of the sarA mutant in terms of proteolytic activity.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:38 CDTGO:0008658 penicillin binding (F)PMID:25257373ECO:0000314 direct assay evidence used in manual assertion

Figure 9 shows the impact of purified aureolysin in contemporary clinical isolates as a function of methicillin resistance status. Methicillin is a member of the penicillin class.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:38 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000314 direct assay evidence used in manual assertion

Figure 10 shows the relative impact of aureolysin and dispersin B on biofilm formation.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:37 CDTGO:0044010 single-species biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 8 shows the difference between biofilm formation in WT and sarA mutants.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:35 CDTGO:1900232 negative regulation of single-species biofilm formation on inanimate substrate (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 7 shows the impact that mutating individual protease genes/operons in vivo has on the ability of WT and its isogenic sarA mutant to produce proteases. The repression of protease production in turn promotes biofilm formation.

challenge
updatedbyinstructorSTAA3:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:34 CDTGO:0090609 single-species submerged biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 6 shows the impact of mutating individual protease genes or operons in both the WT and sarA mutant in terms of biofilm formation.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:32 CDTGO:0098786 biofilm matrix disassembly (P)PMID:25257373ECO:0000314 direct assay evidence used in manual assertion

Figure 5 shows the impact of purified extracellular proteases on dispersal of established biofilms.

challenge
updatedbyinstructorSTAA3:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:30 CDTGO:0045862 proteolysis (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 3 shows the impact of mutating individual protease genes or operons in both the WT and sarA mutant in terms of proteolytic activity.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:30 CDTGO:0090605 submerged biofilm formation (P)PMID:25257373ECO:0000314 direct assay evidence used in manual assertion

Figure 4 shows how purified extracellular proteases impact biofilm formation in LAC protease-deficient derivatives.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:28 CDTGO:0044010 single-species biofilm formation (P)PMID:25257373ECO:0000314 direct assay evidence used in manual assertion

Figure 2 shows the impact of purified extracellular proteases aureolysin, SspA, ScpA, and SspB on biofilm formation.

challenge
unacceptableSTAAU:SARAMargalit.R.Balaban, Team The Herd2016-03-18 17:22:27 CDTGO:0090605 submerged biofilm formation (P)PMID:25257373ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 1 defines the optimal conditions for biofilm formation in vitro for both WT and isogenic sarA mutants.

challenge
unacceptableSTAAU:A0A0M1TZU6Indra.Bhattarai, Team The Herd2016-03-08 17:35:17 CSTGO:0008658 penicillin binding (Antibiotic susceptibility test was conducted using antibiotics according to the guidelines of Clinical and Laboratory Standards Institute. Also,Phenotypic biofilm formation was performed with microtiter plate assay.)PMID:21800213IDA: Inferred from Direct Assay
challenge
unacceptableSTAAU:W8U1E4Jama.Hersi, Team The Herd2016-03-05 18:35:54 CSTGO:0008233 peptidase activity (F)PMID:26890675ECO:0000316 genetic interaction evidence used in manual assertion

Figure 1 shows that the left panel shows the entire complex, whereas the right panel shows a zoomed image of the cephalosporin binding pocket. The Staphylococcus aureus strain depicted in the crystal structure (PDB 3HUM) [30], Col, is the parental strain of the Colnex strain used in the current study (Table 1). Mutant residues in PBP4 identified by selection with ceftaroline or ceftobiprole are highlighted in blue. The ligand marked in yellow is cefotaxime.

challenge
unacceptableSTAAU:A0A0E1X950Indra.Bhattarai, Team The Herd2016-03-05 18:34:28 CSTGO:0016020 membrane (C)PMID:22492855ECO:0000315 mutant phenotype evidence used in manual assertion

figure A. shows Vancomycin minimum inhibitory concentrations (MICs) were determined by Etest for A5937, A5937Δstp1, the complemented strain A5937Δstp1-C, and the vancomycin nonsusceptible isolate A5940. Values in parentheses represent MICs based on broth dilution.Figure B. shows Determination of vancomycin heteroresistance was performed using population analysis profiling. The stp1 deletion mutant had subpopulations that grew in the nonsusceptible range (>2 µg/mL); this was not seen in the parent strain (A5937) but was more pronounced in the clinical vancomycin-intermediate S. aureus daughter strain (A5940). CFU, colony-forming unit. Figure C. shows transmission electron microscopy was performed to determine cell wall thickness. Three representative cells from each strain are shown, and values represent mean cell wall thickness (95% confidence interval).

challenge
unacceptableSTAAU:BLAIJames.R.Workin, Team The Herd2016-02-19 11:53:22 CSTGO:0008800 beta-lactamase activity (F)PMID:26796298ECO:0000314 direct assay evidence used in manual assertion

Table 1. Distribution of various virulence, regulatory and toxin genes.

challenge

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Pages in category "Team The Herd"

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