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Category:Team Grapes of Staph

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableALIFS:LUXRLevi.C.Schlosser, Team Grapes of Staph2016-04-19 11:19:45 CDTGO:0045893 positive regulation of transcription, DNA-templated (P)PMID:1597420ECO:0000314 direct assay evidence used in manual assertion

Fig. 1: the operator and the arrows indicate the direction of transcription from the two characterized of promoters.

challenge
updatedbyinstructorVIBF1:Q5E615Heidi.Larson.1, Team Grapes of Staph2016-04-12 05:10:27 CDTGO:0008218 bioluminescence (P)PMID:24056099ECO:0000316 genetic interaction evidence used in manual assertion

Fig. 2 shows that the ainR mutant has decreased luminescence. This decreased luminescence can be mediated with the addition of C8-AHL. This shows that ainR in V. fischeri acts almost identically to luxN in V. harveyi.

challenge
unacceptableALIFS:Q6WEJ3Nur.Isaak, Team Grapes of Staph2016-04-11 08:58:44 CDTGO:0003677 DNA binding (F)PMID:25962099ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 2 shows the binding regulation of LuxR.

challenge
updatedbyinstructorALIFS:LUXANathan.Marsh, Team Grapes of Staph2016-04-11 07:47:27 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows TEMs of the narrow columnar cells of the LuxA mutant infected squids vs the enlarged columnar cells of the wild type infected squids. The graph also depicts the decreased cytoplasmic volume between the two.

challenge
updatedbyinstructorALIFS:LUXRNathan.Marsh, Team Grapes of Staph2016-04-11 07:31:57 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

The table in Figure 5 shows the decreased levels of cytoplasm in the LuxR mutant vs the uninfected squids and wild type infected squids.

challenge
updatedbyinstructorALIFS:LUXINathan.Marsh, Team Grapes of Staph2016-04-11 07:24:56 CDTGO:0052043 modification by symbiont of host cellular component (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Figure 5 shows the different cytoplasmic volumes of the mutants and uninfected vs the wild type.

challenge
acceptableALIFS:LUXANathan.Marsh, Team Grapes of Staph2016-04-11 05:15:25 CDTGO:0008218 bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that the LuxA gene is necessary for bioluminescence (not a regulator). Figure 2 also shows that biolumiscence is low in the LuxA mutants of the colonized juvenile squids in the experiment.

challenge
updatedbyinstructorVIBF1:Q5E268Heidi.Larson.1, Team Grapes of Staph2016-03-29 03:26:00 CDTGO:0006083 acetate metabolic process (P)PMID:18487321ECO:0000315 mutant phenotype evidence used in manual assertion

Fig 5, Table 2

challenge
unacceptableVIBF1:LUXSHeidi.Larson.1, Team Grapes of Staph2016-03-07 22:56:13 CSTGO:0003700 transcription factor activity, sequence-specific DNA binding (F)other:17827287ECO:0000314 direct assay evidence used in manual assertion

Fig. 2 shows direct binding of LuxR protein to promoters of genes of Lux operon

challenge
acceptableALIFS:LUXRNathan.Marsh, Team Grapes of Staph2016-03-07 17:56:11 CSTGO:1905087 positive regulation of bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 displays results of autoinducing molecules that can trigger the LuxR mutant into bioluminescing. Fig 2 signifies that when the luxR is not present the bioluminescence is significantly decreased in the colonized juvenile squids.

challenge
acceptableALIFS:LUXINathan.Marsh, Team Grapes of Staph2016-03-07 17:52:42 CSTGO:1905087 positive regulation of bioluminescence (P)PMID:10913092ECO:0000315 mutant phenotype evidence used in manual assertion

Table 1 shows that when autoinducers are introduced to the LuxI mutant then the bacteria will bioluminesce. Fig 2 signifies that when the luxI is not present the bioluminescence is significantly decreased in the colonized juvenile squid.

challenge
unacceptableVIBF1:Q5E614Caleb.N.Laney, Team Grapes of Staph2016-03-05 13:39:10 CSTGO:0007165 signal transduction (P)PMID:14507383ECO:0000315 mutant phenotype evidence used in manual assertion

Table 3. Demonstrates how ainS mutants were unable to synthesize C8-HSL, preventing sufficient signalling to repress the LuxO repressor. This phenomenon resulted in a decrease in symbiotic luminescence.

challenge
unacceptableALIFS:LUXIHeidi.Larson.1, Team Grapes of Staph2016-02-22 10:59:37 CSTGO:0061579 N-acyl homoserine lactone synthase activity (F)PMID:17827287ECO:0000317 genomic context evidence used in manual assertion
challenge
unacceptableVIBF1:LUXSCaleb.N.Laney, Team Grapes of Staph2016-02-19 11:51:56 CSTGO:0051101 regulation of DNA binding (P)PMID:22500943ECO:0000314 direct assay evidence used in manual assertion

A microarray study showed that the LuxR protein encoded by the luxR gene is a positive and negative regulator of different genes involved in bioluminescence.

challenge

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Pages in category "Team Grapes of Staph"

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