User:GFurletti

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
updatedbyinstructor	YERPE:Q93NB6	2019-03-08 15:43:31 CST	GO:0019662 non-glycolytic fermentation (P)	PMID:25220241	ECO:0006049 genetic transformation evidence used in manual assertion	Table 2: Two different strains of Y. pestis, KIM6+, with a functional glpD gene, and CO92L, with a 93 bp inframe deletion of the glpD allele, were tested for glycerol fermentation under differing conditions. Glycerol fermentation was visualized by indicator plates. Unmodified strains were utilized as controls, in which KIM6+ was positive for glycerol fermentation and CO92L was negative for glycerol fermentation. Allelic exchange was used to exchange the functional glpD in KIM6+ with the nonfunctional glpD of CO92L. The nonfunctional glpD of CO92L was exchanged with the functional glpD of KIM6+. KIM6+ with nonfunctional glpD and CO92L with the functional glpD showed no indicators of glycerol fermentation. Glycerol fermentation was restored in the mutant KIM6+ with nonfunctional glpD when a plasmid vector of functional glpD derived from KIM6+ was added. This is evidence lack of glpD can disrupt glycerol fermentation. GO Term non-glycolytic fermentation was chosen due to the function of glpD. glpD in this experiment participates in aerobic glycerol fermentation to help form pyruvate from glycerol which does not involve the use of glycolysis as in glycolysis fermentation.	challenge

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Annotations challenged by GFurletti

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
acceptable	VMahaney, Team Red A 2019	ECOLX:A0A1W5T566	GO:0020035 - adhesion of symbiont to microvasculature (P)	PMID:30845261	ECO:0001143 adhesion assay evidence used in manual assertion	challenge	C: 2
acceptable	SLudwig, Team Red A 2019	ECOLX:A0A1W5T566	GO:0034446 - substrate adhesion-dependent cell spreading (P)	PMID:30845261	ECO:0007046 scanning electron microscopy evidence used in manual assertion	challenge	C: 2

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