ECOLX:A0A1W5T566

Species (Taxon ID)	Escherichia coli. (562)
Gene Name(s)	cnf1 (ECO:0000313 with EMBL:ARF06329.1)
Protein Name(s)	Cytotoxic necrotizing factor 1 (ECO:0000313 with EMBL:ARF06329.1)
External Links
UniProt	A0A1W5T566
EMBL	KX446780
GO	GO:0016021

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:2000251	positive regulation of actin cytoskeleton reorganization	PMID:30845261	ECO:0006062	wide-field fluorescence microscopy evidence used in manual assertion		P		In Figure 4 they use florescence microscopy to confirm the CNF1 protein in E.coli induces changes in the organization of the actin cytoskeleton in stress fibers and membrane protrusions, which prevents cytoadherence. This is shown through the addition of CNF1 protein and other inhibitors. With the addition of CNF1 compared to the control you can see the rearrangement of the cytoskeleton but, in the presence of inhibitors along with CNF1 the modifications of rearrangement are decreased.	complete CACAO 13556
	GO:0020035	cytoadherence to microvasculature, mediated by symbiont protein	PMID:30845261	ECO:0001143	adhesion assay evidence used in manual assertion		P		In Figure 1, adhesion assays were used to determine that CNF1 decreased pRBC cytoadherence in both cell line models by at least 40 percent. This is proven by comparing the pre- and post-treatment of the CNF1 protein with the TNF-alpha activated control. In figure 1A, CNF1 is used as pre-treatment to prevent the attachment of pRBC. In figure 1B, CNF1 is used as post-treatment to reverse adherence of pRBCs to endothelial cells.	complete CACAO 13658
	GO:0034446	substrate adhesion-dependent cell spreading	PMID:30845261	ECO:0007046	scanning electron microscopy evidence used in manual assertion		P		Figure 3A is a scanning electron micrograph that shows the response of brain cells to treatment with CNF1 protein, a mutant version of the protein (CNF1 C866S), or a control (TNF-alpha). Cells treated with CNF1 had increased monolayer confluence, as CNF1 promoted cell spreading and flattening. Cells treated with CNF1 C866S or TNF-alpha had an unchanged surface morphology in comparison.	complete CACAO 13721
part_of	GO:0016020	membrane	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0472	C		Seeded From UniProt	complete
part_of	GO:0016021	integral component of membrane	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0812	C		Seeded From UniProt	complete
edit table

References

See Help:References for how to manage references in GONUTS.