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CACAO Phage Hunters 2019

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
acceptableECOLX:A0A1W5T5662019-03-08 18:42:46 CSTGO:2000251 positive regulation of actin cytoskeleton reorganization (P)PMID:30845261ECO:0006062 wide-field fluorescence microscopy evidence used in manual assertion

In Figure 4 they use florescence microscopy to confirm the CNF1 protein in E.coli induces changes in the organization of the actin cytoskeleton in stress fibers and membrane protrusions, which prevents cytoadherence. This is shown through the addition of CNF1 protein and other inhibitors. With the addition of CNF1 compared to the control you can see the rearrangement of the cytoskeleton but, in the presence of inhibitors along with CNF1 the modifications of rearrangement are decreased.

acceptableECOLX:A0A1W5T5662019-03-29 11:22:18 CDTGO:0020035 cytoadherence to microvasculature, mediated by symbiont protein (P)PMID:30845261ECO:0001143 adhesion assay evidence used in manual assertion

In Figure 1, adhesion assays were used to determine that CNF1 decreased pRBC cytoadherence in both cell line models by at least 40 percent. This is proven by comparing the pre- and post-treatment of the CNF1 protein with the TNF-alpha activated control. In figure 1A, CNF1 is used as pre-treatment to prevent the attachment of pRBC. In figure 1B, CNF1 is used as post-treatment to reverse adherence of pRBCs to endothelial cells.



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