User:Dahle001

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
unacceptable	STAAU:MECR	2012-02-23 12:25:23 CST	GO:0016481 negative regulation of transcription (1)	PMID:22295022	ECO:0000314 direct assay evidence used in manual assertion	Figure 1, part A1	challenge
requireschanges	HUMAN:ATF4	2012-03-12 15:37:31 CDT	GO:0010467 gene expression (P)	PMID:22363646	ECO:0000314 direct assay evidence used in manual assertion	Figure 1A: Protein and mRNA levels of ATF4 were determined using Western Blot and qPCR. β-actin and GAPDH were used as a control.	challenge
requireschanges	HUMAN:ATF4	2012-03-12 15:58:54 CDT	GO:0001558 regulation of cell growth (P)	PMID:22363646	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 1B, C, D, E, H: When transfected into SGC7901 and AGS cells, colony number increased due to overexpression of ATF4. When ATF4 was knocked out of expression in SGC7901/ADR and SGC7901/VCR cells the colony number decreased. This shows that ATF4 produced multi-drug resistance in cancer cells, allowing for cell growth.	challenge
requireschanges	HUMAN:SIRT1	2012-03-13 14:28:15 CDT	GO:0042493 response to drug (P)	PMID:22363646	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4: Knockdown of SIRT1 allows the cells to become sensitive to the drug. Data shows that SIRT1 allows the cell to be drug resistant.	challenge
requireschanges	HUMAN:SIRT1	2012-03-13 14:37:29 CDT	GO:0043066 negative regulation of apoptotic process (P)	PMID:22363646	ECO:0000270 expression pattern evidence used in manual assertion	Figure 5A-C: overexpression of SIRT1 allows for the downregulation of apoptosis, allowing for ATF4-induced multi-drug resistance in gastric cancer.	challenge
acceptable	RAT:FAS	2012-04-10 12:38:33 CDT	GO:0004312 fatty acid synthase activity (F)	PMID:21389266	ECO:0000314 direct assay evidence used in manual assertion	Figure 1	challenge

acceptable:1
unacceptable:1
requires_changes:4
flagged:0

Annotations challenged by Dahle001

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
requireschanges	Yuen0005, Team Rico FTW	HUMAN:SRC	GO:0004715 - non-membrane spanning protein tyrosine kinase activity (F)	PMID:22303479	ECO:0000314 direct assay evidence used in manual assertion	challenge	C: 1

0 annotations fixed by Dahle001