User:Acmurthy

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
requireschanges	CLOPF:G5DS78	2015-03-20 15:08:00 CDT	GO:0043684 type IV secretion system complex (C)	PMID:25488300	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 1B shows the conjugation frequency of a mutant lacking tcpE. tcpE is complemented to restore mating.	challenge
requireschanges	CLOPF:G5DS78	2015-03-20 15:08:00 CDT	GO:0030313 cell envelope (C)	PMID:25488300	ECO:0000314 direct assay evidence used in manual assertion	In Fig. 2, TcpE was found in the cell lysate and insoluble membrane fraction.	challenge
requireschanges	CLOPF:G5DS78	2015-03-20 15:08:00 CDT	GO:0060187 cell pole (C)	PMID:25488300	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4 shows TcpE localizing to the cell poles.	challenge
requireschanges	CLOPF:G5DSP0	2015-03-20 15:08:19 CDT	GO:0043684 type IV secretion system complex (C)	PMID:25488300	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 1B shows the conjugation frequency of a mutant lacking tcpD. tcpD is complemented to restore mating.	challenge
requireschanges	CLOPF:G5DSP0	2015-03-20 15:08:19 CDT	GO:0030313 cell envelope (C)	PMID:25488300	ECO:0000314 direct assay evidence used in manual assertion	In Fig. 2, TcpD was found in the cell lysate and insoluble membrane fraction.	challenge
requireschanges	CLOPF:G5DSP0	2015-03-20 15:08:19 CDT	GO:0060187 cell pole (C)	PMID:25488300	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4 shows TcpD localizing to the cell poles.	challenge
correctedthroughchallenges	STRAZ:Q8GGN5	2015-03-20 15:23:54 CDT	GO:0019184 nonribosomal peptide biosynthetic process (P)	PMID:15112993	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 4B shows HPLC analysis of the formation of leinamycin. Mutants lacking lnmQ don't produce leinamycin.	challenge
acceptable	STRAZ:Q8GGN5	2015-03-20 15:23:54 CDT	GO:0043042 amino acid adenylylation by nonribosomal peptide synthase (P)	PMID:17502372	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows the amino acid specificity and Michaelis-Menton kinetics of LnmQ.	challenge
acceptable	STRAZ:Q8GGP1	2015-03-20 15:31:20 CDT	GO:0016417 S-acyltransferase activity (F)	PMID:23320975	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 6 shows trans-acylation of LnmL (ACP-SH) by LnmK. Active site mutants lose this ability.	challenge
requireschanges	ENTFL:D1LHF5	2015-03-20 16:30:05 CDT	GO:0009253 peptidoglycan catabolic process (P)	PMID:24244005	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2,3, and 4 show that PrgK PG hydrolase domains are active in vivo and in vitro.	challenge
requireschanges	ENTFL:D1LHF5	2015-03-20 16:30:05 CDT	GO:0043684 type IV secretion system complex (C)	PMID:24244005	ECO:0000315 mutant phenotype evidence used in manual assertion	Fig. 1 shows that PrgK and its PG hydrolase domains are required for conjugation.	challenge
requireschanges	ENTFL:E3WD67	2015-03-20 16:49:48 CDT	GO:0043684 type IV secretion system complex (C)	PMID:23913323	ECO:0000315 mutant phenotype evidence used in manual assertion	Mating assays with a traG deletion mutant resulted in no mating as compared to wild-type.	challenge
requireschanges	ENTFL:E3WD67	2015-03-20 16:49:48 CDT	GO:0009253 peptidoglycan catabolic process (P)	PMID:23913323	ECO:0000314 direct assay evidence used in manual assertion	Fig. 4 shows Cy3 PG degradation by TraGdTMH.	challenge
requireschanges	ENTFL:E3WD67	2015-03-20 16:49:48 CDT	GO:0030313 cell envelope (C)	PMID:23913323	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3 shows that TraG is found in the cell wall and membrane fraction.	challenge
requireschanges	ECOLI:TRAB1	2015-03-20 17:26:39 CDT	GO:0032993 protein-DNA complex (C)	PMID:20172994	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows the interaction between different TraB domains and DNA using a gel shift assay.	challenge
requireschanges	ECOLI:TRAB1	2015-03-20 17:26:39 CDT	GO:0005524 ATP binding (F)	PMID:20172994	ECO:0000314 direct assay evidence used in manual assertion	Fig. 3 shows TraB and TraB fragment bind to a fluorescent analog of ATP.	challenge
acceptable	ECOLI:TRAB1	2015-03-20 17:26:40 CDT	GO:0016887 ATPase activity (F)	PMID:20172994	ECO:0000314 direct assay evidence used in manual assertion	Table 2 and Fig. 5 show the ATPase activity of TraB and analysis using Michaelis-Menton kinetics.	challenge
requireschanges	HUMAN:DNMT1	2015-03-21 16:57:52 CDT	GO:0042127 regulation of cell proliferation (P)	PMID:21572997	ECO:0000314 direct assay evidence used in manual assertion	FIg. 2B shows that inhibition of DNMTs decreases the proliferation rate in a dose-dependent manner. Fig. 2E shows that inhibition of DNMTs arrests most cells in the G1 phase.	challenge
requireschanges	HUMAN:DNM3A	2015-03-21 17:04:59 CDT	GO:0042127 regulation of cell proliferation (P)	PMID:21572997	ECO:0000314 direct assay evidence used in manual assertion	FIg. 2B shows that inhibition of DNMTs decreases the proliferation rate in a dose-dependent manner. Fig. 2E shows that inhibition of DNMTs arrests most cells in the G1 phase.	challenge
requireschanges	HUMAN:DNM3B	2015-03-21 17:08:17 CDT	GO:0042127 regulation of cell proliferation (P)	PMID:21572997	ECO:0000314 direct assay evidence used in manual assertion	FIg. 2B shows that inhibition of DNMTs decreases the proliferation rate in a dose-dependent manner. Fig. 2E shows that inhibition of DNMTs arrests most cells in the G1 phase.	challenge
acceptable	ENTFL:Q5G3P5	2015-03-22 16:47:59 CDT	GO:0016887 ATPase activity (F)	PMID:22636769	ECO:0000314 direct assay evidence used in manual assertion	Fig. 2 shows the ATPase activity of PrgJ fitted to the Michaelis-Menton equation.	challenge

acceptable:4
unacceptable:0
requires_changes:16
flagged:0

Annotations challenged by Acmurthy

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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