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PMID:25488300
| Citation |
Wisniewski, JA, Teng, WL, Bannam, TL and Rood, JI (2015) Two novel membrane proteins, TcpD and TcpE, are essential for conjugative transfer of pCW3 in Clostridium perfringens. J. Bacteriol. 197:774-81 |
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| Abstract |
The anaerobic pathogen Clostridium perfringens encodes either toxin genes or antibiotic resistance determinants on a unique family of conjugative plasmids that have a novel conjugation region, the tcp locus. Studies of the paradigm conjugative plasmid from C. perfringens, the 47-kb tetracycline resistance plasmid pCW3, have identified several tcp-encoded proteins that are involved in conjugative transfer and form part of the transfer apparatus. In this study, the role of the conserved hypothetical proteins TcpD, TcpE, and TcpJ was examined. Mutation and complementation analyses showed that TcpD and TcpE were essential for the conjugative transfer of pCW3, whereas TcpJ was not required. To analyze the TcpD and TcpE proteins in C. perfringens, functional hemagglutinin (HA)-tagged derivatives were constructed. Western blots showed that TcpD and TcpE localized to the cell envelope fraction independently of the presence of other pCW3-encoded proteins. Finally, examination of the subcellular localization of TcpD and TcpE by immunofluorescence showed that these proteins were concentrated at both poles of C. perfringens donor cells, where they are postulated to form essential components of the multiprotein complex that comprises the transfer apparatus. |
| Links |
PubMed PMC4334191 Online version:10.1128/JB.02466-14 |
| Keywords |
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| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0043684: type IV secretion system complex |
ECO:0000315: |
C |
Fig. 1B shows the conjugation frequency of a mutant lacking tcpD. tcpD is complemented to restore mating. |
complete | |||
| GO:0030313: cell envelope |
ECO:0000314: |
C |
In Fig. 2, TcpD was found in the cell lysate and insoluble membrane fraction. |
complete | ||||
| GO:0060187: cell pole |
ECO:0000314: |
C |
Fig. 4 shows TcpD localizing to the cell poles. |
complete | ||||
|
Contributes to |
GO:0043684: type IV secretion system complex |
ECO:0000315: |
C |
Fig. 1B shows the conjugation frequency of a mutant lacking tcpE. tcpE is complemented to restore mating. |
complete | |||
| GO:0030313: cell envelope |
ECO:0000314: |
C |
In Fig. 2, TcpE was found in the cell lysate and insoluble membrane fraction. |
complete | ||||
| GO:0060187: cell pole |
ECO:0000314: |
C |
Fig. 4 shows TcpE localizing to the cell poles. |
complete | ||||
Notes
See also
References
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