Cacao

Yes, the notes are a bit long, but there's nothing technically incorrect about long notes. Also, I already noted that the publication was missing, so you'd have to fix a problem to get any points.

This annotation has been flagged because it has been edited since last assessment ||GO:0019046||release from viral latency||PMID:||IMP: Inferred from Mutant Phenotype||||P||BRLF1 is a transcriptional activator that mediates the switch from latent to lytic viral replication.

Figure 1 A-C: Three different cell lines were transfected with plasmids for the over-expression of Na (which is the product of gene BRRF1) as well as control plasmids with no over-expression of Na. To see if Na initiated lysis in the cells in which Na was overproduced, immunoassays were performed to search for lytic cell products. The proteins searched for include BMRF1, Z protein, R protein, Na (through FLAG), and β-actin. In all three types of cells with plasmids over-expressing BRRF1, these lytic proteins were found in a greater amount than in the cells without the over-expression plasmids. Expression of BRRF1 leads to a product that affects maintenance of viral latency. The evidence code is IMP because there is over-expression of the gene. Presence of the gene product resulted in an increase in specific lytic proteins measured by an immunoblot assay.

||Missing: reference