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Cacao
| GO:2000892 | cellobiose catabolic process | PMID:28118504 | ECO:0001175 IMP: Inferred from Mutant Phenotype | Biological Process | Mutant strains with individual and combinatorial mutations were generated and growth assays were conducted to determine which genes were essential for allowing Cellvibrio japonicus to metabolize cellobiose, a disaccharide that composes cellulose. According to Figure 1A, cel3B deletion most significantly hinders growth of C. japonicus on cellobiose. Figure 2D also showed that the cel3B single mutants (triangles) had decreased cellobiose consumption and a longer lag phase compared to the wild type (circles). When cel3B was introduced into E. coli (figure 3B), which normally cannot degrade cellulose or its constituents, E. coli was able to grow on cellobiose media albeit slowly. This shows that the gene product of cel3B, an enzyme, allows the cell to use cellobiose as a carbon source. | complete | ||
| This annotation made on page: CELJU:B3PDN7 By: CShee (group Team Green B 2019) on 2019-03-31 11:02:19 CDT. | ||||||||
You must be logged in to challenge this annotation.
| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Challenge | KAtanasoff, Team Blue A 2019 | 2019-04-01 10:12:08 CDT | You use an ECO term for deletion mutation evidence, but also cite genetic recombination evidence (i.e. the data from figure 3B). This should probably be split up into multiple annotations, each providing evidence that the Cel3B protein is involved with cellobiose catabolism. | 1 | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-04-14 13:20:51 CDT | No notes given. | Acceptable ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-04-09 09:44:42 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged | ||||||||||||||||||||||
| Public Assessment | Ivanerill | 2019-04-08 16:56:21 CDT | The authors are knocking out genes, not deleting a few bases from them. There is a proper ECO term for that. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✗ Evidence ✔ With/From ✔ Notes ✔ Unique/Original | ||||||||||||||||||||||
| Public Assessment | DanielRenfro | 2019-04-03 11:58:29 CDT | This annotation has been flagged because it has been edited since last assessment
| Flagged |
