User:CShee

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	STRCO:CYC2	2019-03-10 17:26:08 CDT	GO:0044550 secondary metabolite biosynthetic process (P)	PMID:12563033	ECO:0006091 functional complementation evidence used in manual assertion	In this paper, the researchers used PCR targeting, gene replacement cassette, and transposon mutagenesis to determine whether genes cyc1 and cyc2 were both required for production of geosmin, a substance that gives soil its distinctive scent. GC-MS was used to detect geosmin production by each mutant. In Table 3, it is shown that when cyc2 is deleted, (strains J3001, J3002) there is no production of geosmin. When cyc1 is mutated using a transposon, geosmin is still produced. This indicates that cyc2, not cyc1, is required for the production of geosmin. To confirm this, the researchers reintroduced the cyc2 gene (complementation) via a vector into the strain, and geosmin production was restored.	challenge
acceptable	CELJU:B3PDN7	2019-03-31 11:02:19 CDT	GO:2000892 cellobiose catabolic process (P)	PMID:28118504	ECO:0001225 knockout evidence used in manual assertion	Mutant strains with individual and combinatorial mutations were generated and growth assays were conducted to determine which genes were essential for allowing Cellvibrio japonicus to metabolize cellobiose, a disaccharide that composes cellulose. According to Figure 1A, cel3B deletion most significantly hinders growth of C. japonicus on cellobiose. Figure 2D also showed that the cel3B single mutants (triangles) had decreased cellobiose consumption and a longer lag phase compared to the wild type (circles). This shows that the gene product of cel3B, an enzyme, helps the cell use cellobiose as a carbon source.	challenge

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unacceptable:0
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Annotations challenged by CShee

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history

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