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GO:0019835cytolysisPMID:26375388IDA: Inferred from Direct Assay P
This annotation made on page: 9VIRU:A0A088FLK9
By: DFrank (group Team Green A 2018) on 2018-03-17 18:09:40 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeMKoert,
Team Red A 2018
2018-03-23 14:12:09 CDT

I am not sure I agree with your GO term, the evidence you shown definitely points to it being lytic and it is likely your gene is an endolysin however I am not sure it meets the specific cytolysis definition of "The rupture of cell membranes and the loss of cytoplasm" without showing it actively ruptures the cytoplasm, just showing that it has the same mass as an endolysin and it kills the cells. Perhaps try a different GO term would be better suited or possibly more data showing that it works by breaking the cytoplasm.

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JimHu2019-06-06 16:18:28 CDTYou need to be an instructor to view these notes.Updated by Instructor
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JimHu2019-06-06 16:08:57 CDTYou need to be an instructor to view these notes.Requires Changes
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Ivanerill2018-05-07 08:18:23 CDTNo notes given.Acceptable
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DanielRenfro2018-05-02 13:37:07 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
Contributes to GO:0051715 cytolysis in other organism PMID:26375388 IDA: Inferred from Direct Assay P In figure 3, T. thermophilus was exposed to different concentrations of Ts2631 endolysin. At 5 minute intervals, for 40 minutes, the number of cells still alive was found by measuring OD600. According to the graph, no matter what concentration of endolysin was added, a significant number of cells died compared to cell death in the control. At the 35 minute mark, all concentration amounts showed over 50% cell death. This means that the endolysin from Ts2631 phage causes the death of the host cell. complete
CACAO 13124
on 9VIRU:A0A088FLK9
Flagged
Public
Assessment
Ivanerill2018-05-02 11:44:39 CDT

no, that is not a term derived from GO:0019835. Go to QuickGO and look at GO:0019835 descendents. Think about which organism cell is being lysed

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DanielRenfro2018-05-02 11:01:38 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
Contributes to GO:0031343 positive regulation of cell killing PMID:26375388 IDA: Inferred from Direct Assay P In figure 3, T. thermophilus was exposed to different concentrations of Ts2631 endolysin. At 5 minute intervals, for 40 minutes, the number of cells still alive was found by measuring OD600. According to the graph, no matter what concentration of endolysin was added, a significant number of cells died compared to cell death in the control. At the 35 minute mark, all concentration amounts showed over 50% cell death. This means that the endolysin from Ts2631 phage causes the death of the host cell. complete
CACAO 13124
on 9VIRU:A0A088FLK9
Flagged
Public
Assessment
Ivanerill2018-04-30 05:51:52 CDT

Your notes: "This means that the endolysin from Ts2631 phage causes the death of the host cell." Your initial GO term: GO:0019835 - cytolysis. Find a derived term that captures the gist of your note (regulation of cytolysis is not it).

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DanielRenfro2018-04-15 15:22:28 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
Contributes to GO:0045919 positive regulation of cytolysis PMID:26375388 IDA: Inferred from Direct Assay P In figure 3, T. thermophilus was exposed to different concentrations of Ts2631 endolysin. At 5 minute intervals, for 40 minutes, the number of cells still alive was found by measuring OD600. According to the graph, no matter what concentration of endolysin was added, a significant number of cells died compared to cell death in the control. At the 35 minute mark, all concentration amounts showed over 50% cell death. This means that the endolysin from Ts2631 phage causes the death of the host cell. complete
CACAO 13124
on 9VIRU:A0A088FLK9
Flagged
Public
Assessment
Ivanerill2018-04-14 19:38:02 CDT

"T. thermophilus was infected with different concentrations of Ts2631 endolysin." - exposing a cell to a lysin is not "infecting" it; rephrase There is not infection, so the experiment does not prove "killing by virus of host cell" Go back to the https://gowiki.tamu.edu/wiki/index.php/Category:virus-related_terms page or QuickGO to find a term that derives from your original GO:0019835 and applies better to phages

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DanielRenfro2018-04-13 20:08:24 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0039633 killing by virus of host cell PMID:26375388 IDA: Inferred from Direct Assay P In figure 3, T. thermophilus was infected with different concentrations of Ts2631 endolysin. At 5 minute intervals, for 40 minutes, the number of cells still alive was found by measuring OD600. According to the graph, no matter what concentration of endolysin was added, a significant number of cells died compared to cell death in the control. At the 35 minute mark, all concentration amounts showed over 50% cell death. This means that the endolysin from Ts2631 phage causes the death of the host cell. complete
CACAO 13124
on 9VIRU:A0A088FLK9
Flagged
Public
Assessment
Ivanerill2018-03-24 12:39:16 CDT

Why point to a supplementary figure when there is a figure in the manuscript demonstrating of Ts2631 endolysin lytic activity? Check https://gowiki.tamu.edu/wiki/index.php/Category:virus-related_terms for better suited GO terms.

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