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Cacao

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GO:0020002host cell plasma membranePMID:27825035IDA: Inferred from Direct Assay C
This annotation made on page: BPSPP:O48472
By: Mcgillje (group Team Phergalicious) on 2017-04-05 10:07:16 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeRamaswamya,
Team North Phace
2017-04-12 09:47:28 CDT

First off you should specify which part of the figure are you talking: about the top two images showed SDS page analysis while the bottom two images showed Western Blot analysis of two different proteins gp 24.1 and 26. You should probably analyze the presence of a faint third band in figure D under the cytoplasmic section of the blot.

None, yet.
ChallengeSyedsa,
Team Viridae
2017-04-10 12:09:28 CDT

Addition of information on the localized membranes of the control, gp24.1, and gp26 could be used to strengthen annotation on "protein product was detected at much higher levels in the cell membrane, as opposed to the cytoplasm," as it is unclear on what figure 4 represents.

None, yet.
Private
Assessment
AAJohnson2017-05-07 17:47:14 CDTYou need to be an instructor to view these notes.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2017-04-19 09:36:35 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0020002 host cell plasma membrane PMID:27825035 IDA: Inferred from Direct Assay C In Figure 4, the membrane fraction column (M) of the Western blot panel (bottom row) for gp26 shows that the protein product was detected at much higher levels in the cell membrane, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp26. complete
CACAO 12436
on BPSPP:O48472
Flagged
Public
Assessment
DanielRenfro2017-04-19 09:14:07 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0020002 host cell plasma membrane PMID:27825035 IDA: Inferred from Direct Assay C Figure 4 shows that the protein product was detected at much higher levels in the cell membrane, as opposed to the cytoplasm. Cell fractionation into cytoplasmic and membrane fractions followed by Western blot shows a thicker band of protein in the membrane fraction of the cells expressing gp26. complete
CACAO 12436
on BPSPP:O48472
Flagged