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GO:0044085cellular component biogenesisPMID:27013840IDA: Inferred from Direct Assay P
This annotation made on page: ENTFA:GELE
By: Mitchell.L.Kennedy (group Team Spartan) on 2016-03-29 03:17:48 CDT.



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Ebcaraway2016-04-05 16:16:25 CDT

Okay, so here is a case where the paper you choose looked like you could pull a good annotation out, but you can't. Let's take a look at how they conducted this study. The researchers were interested in seeing if there was a correlation between known virulence genes and and vancomycin resistance in Enterococci. So here it's important to remember that Enterococci is a very large genus. They took samples from a variety of bodily fluids from patients and cultured, put through a battery of phenotypic tests, checked for antibiotic susceptibility/resistance, and then put through multiplex PCR for the detection for known virulence genes and vancomycin resistance genes.

You annotated to Enterococcus faecalis, which they did find in this study. Of the vancomycin resistant strains (n=12) of this bacterium found, only 4 of them had gelE. Though the authors declared this correlative, especially in conjunction with the other virulence genes sequenced, they did not show that this was related. Mostly, this just seems like you are reaching because you want to annotate as a 'response to vancomycin' and we won't let you.

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