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GO:0006355regulation of transcription, DNA-templatedPMID:23952475IMP: Inferred from Mutant Phenotype P
This annotation made on page: LISMO:CSPA
By: Sydney.B.Bork (group Team Alpha) on 2016-02-19 11:50:09 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeSydney.B.Bork,
Team Alpha
2016-04-15 11:06:54 CDT

The GO term can go further to GO:0019836 hemolysis by symbiont of host erythrocytes, since the cspABD genes affect listeriolysin O which affects the lysis of cells on blood agar (erythrocytes).

None, yet.
ChallengeSydney.B.Bork,
Team Alpha
2016-04-13 10:18:52 CDT

The GO ID should be GO:0044179 for the hemolysis in other organisms.

None, yet.
ChallengeSydney.B.Bork,
Team Alpha
2016-04-13 10:15:34 CDT

The experiment was done with a triple mutant with the cspABD. The evidence should be IGI using with/from P0A357 for cspB and with/from Q92AD0 for cspD.

2
ChallengeNathan.Marsh,
Team Grapes of Staph
2016-03-08 22:15:00 CST

The evidence code should be IGI: Inferred from Mutant Phenotype. This is because the researchers experimented with mutants with multiple knocked out genes and they had stated that the single knock outs did not produce any significant data. The knock out cspABD mutant experienced lower levels of hemolysis compared to the Wt, double deletion mutants of cspAB and cspBD produced similarly significantly lower levels of hemolysis, double deletion mutant cspAD (that retained cspB) produced similar hemolysis as the Wt.

2
Private
Assessment
Suzialeksander2016-06-13 13:29:42 CDTYou need to be an instructor to view these notes.Updated by Instructor
Private
Assessment
Suzialeksander2016-05-03 16:32:44 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2016-04-15 11:06:54 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment
DanielRenfro2016-04-13 10:18:52 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment
DanielRenfro2016-04-13 10:15:34 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2016-04-11 17:02:50 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Public
Assessment
DanielRenfro2016-03-23 11:19:51 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006355 regulation of transcription, DNA-templated PMID:23952475 IGI: Inferred from Genetic Interaction UniProtKB:P0A355 P Csp's are multifunctional nucleic acid binding proteins that regulate gene expression in L. monocytogenes.Lysteriolysin O (LLO) is a pore-forming lysin that allows L. monocytogenes to escape from intracellular vacuoles. LLO is subjected to Csp-dependent regulation. Fig. 1 shows difference in hemolysis in the wild-type and cspABD L. monocytogenes species. complete
CACAO 11279
on LISMO:CSPA
Flagged
Public
Assessment
DanielRenfro2016-03-23 11:12:46 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006355 regulation of transcription, DNA-templated PMID:23952475 IGI: Inferred from Genetic Interaction PMID:23952475 P Csp's are multifunctional nucleic acid binding proteins that regulate gene expression in L. monocytogenes.Lysteriolysin O (LLO) is a pore-forming lysin that allows L. monocytogenes to escape from intracellular vacuoles. LLO is subjected to Csp-dependent regulation. Fig. 1 shows difference in hemolysis in the wild-type and cspABD L. monocytogenes species. complete
CACAO 11279
on LISMO:CSPA
Flagged
Public
Assessment
DanielRenfro2016-03-08 22:15:00 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2016-03-02 14:23:37 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Evidence
With/From
Notes
Unique/Original