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GO:1902495transmembrane transporter complexPMID:22685130IMP: Inferred from Mutant Phenotype C
This annotation made on page: PSEPU:Q9L4M5
By: Roofnich (group MichSt14A 45) on 2014-04-06 17:22:06 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeRoofnich,
MichSt14A 45
2014-04-13 17:53:19 CDT

I'm putting forth a much stronger notes section; this should show why I've assigned the function that I have.

Table 5 shows the ability of E. coli W3110 to oxygenate large hydrophobic substrates (emphasis in the text was on dodecanoic acid methyl ester, referred to as DAME) given differing plasmids from P. putida with different expression levels of alkL and alkBGT. The authors drew the conclusion of AlkL being involved in transporting DAME through the outer membrane based off the difference seen between pBT10 and pBTL10 as both demonstrated high expression of AlkBGT (which is shown to catalyze fatty acid methyl esters in Fig. 1) while pBT10 did not express AlkL and pBTL10 expressed AlkL highly.

0
ChallengeBonifera,
MichSt14A 27
2014-04-09 22:59:29 CDT

While your note is correct, it doesn't seem as if the referenced figure provides a basis for a molecular function of a transmembrane transport protein. The paper repeatedly says that alkL enhances bioconversion by oxygenation, but no evidence is provided that shows that alkL is an actual transport protein.

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Private
Assessment
Suzialeksander2014-05-21 16:42:55 CDTYou need to be an instructor to view these notes.Unacceptable
Private
Assessment
Mill20342014-04-13 10:22:13 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original