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SCHPO:RAD50

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Species (Taxon ID) Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812)
Gene Name(s) rad50
Protein Name(s) DNA repair protein rad50
External Links
UniProt Q9UTJ8
EMBL CU329670
PIR T50080
RefSeq XP_001713090.2
SMR Q9UTJ8
BioGrid 278131
DIP DIP-52389N
IntAct Q9UTJ8
MaxQB Q9UTJ8
PRIDE Q9UTJ8
EnsemblFungi SPAC1556.01c.1
GeneID 2541635
KEGG spo:SPAC1556.01c
EuPathDB FungiDB:SPAC1556.01c
PomBase SPAC1556.01c
HOGENOM HOG000192269
InParanoid Q9UTJ8
KO K10866
OMA RDKYQQE
OrthoDB EOG092C0FLY
PhylomeDB Q9UTJ8
Reactome [www.reactome.org/content/detail/R-SPO-2559586 R-SPO-2559586]
[www.reactome.org/content/detail/R-SPO-5693548 R-SPO-5693548]
[www.reactome.org/content/detail/R-SPO-5693565 R-SPO-5693565]
PRO PR:Q9UTJ8
Proteomes UP000002485
GO GO:0000794
GO:0030870
GO:0000790
GO:0000784
GO:0035861
GO:0004017
GO:0005524
GO:0004003
GO:0003691
GO:0051880
GO:0046872
GO:0043047
GO:0070192
GO:0032508
GO:1990918
GO:0000724
GO:0031573
GO:0042138
GO:1990426
GO:0010520
GO:0016233
GO:0000723
GO:0000722
GO:0007004
InterPro IPR027417
IPR004584
IPR013134
SUPFAM SSF52540
TIGRFAMs TIGR00606
PROSITE PS51131

Annotations

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0547

F

Seeded From UniProt

GO:0000722

telomere maintenance via recombination

PMID:18160711[1]

IGI: Inferred from Genetic Interaction

PomBase:SPAC16A10.07c,PomBase:SPBC29A3.14c

P

Seeded From UniProt

GO:0000723

telomere maintenance

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004584

P

Seeded From UniProt

GO:0000723

telomere maintenance

PMID:12861005[2]

IGI: Inferred from Genetic Interaction

PomBase:SPAC16A10.07c

P

Seeded From UniProt

GO:0000724

double-strand break repair via homologous recombination

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

P

Seeded From UniProt

GO:0000784

nuclear chromosome, telomeric region

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

C

Seeded From UniProt

GO:0000790

nuclear chromatin

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

C

Seeded From UniProt

GO:0000790

nuclear chromatin

GO_REF:0000111

IC: Inferred by Curator

GO:0030870

C

Seeded From UniProt

GO:0000794

condensed nuclear chromosome

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

C

Seeded From UniProt

GO:0003691

double-stranded telomeric DNA binding

GO_REF:0000024

ISO: Inferred from Sequence Orthology

SGD:S000005194

F

Seeded From UniProt

GO:0003691

double-stranded telomeric DNA binding

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

GO:0004003

ATP-dependent DNA helicase activity

GO_REF:0000024

ISO: Inferred from Sequence Orthology

UniProtKB:Q92878

F

Seeded From UniProt

GO:0004017

adenylate kinase activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

GO:0005524

ATP binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0067

F

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004584

C

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0539

C

Seeded From UniProt

GO:0005634

nucleus

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0191

C

Seeded From UniProt

GO:0005694

chromosome

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0158

C

Seeded From UniProt

GO:0005694

chromosome

GO_REF:0000039

IEA: Inferred from Electronic Annotation

UniProtKB-SubCell:SL-0468

C

Seeded From UniProt

GO:0006281

DNA repair

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004584

P

Seeded From UniProt

GO:0006281

DNA repair

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0234

P

Seeded From UniProt

GO:0006974

cellular response to DNA damage stimulus

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0227

P

Seeded From UniProt

GO:0007004

telomere maintenance via telomerase

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

P

Seeded From UniProt

GO:0010520

regulation of reciprocal meiotic recombination

PMID:11726502[3]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0016233

telomere capping

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

P

Seeded From UniProt

GO:0016787

hydrolase activity

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0378

F

Seeded From UniProt

GO:0016887

ATPase activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004584

F

Seeded From UniProt

GO:0030870

Mre11 complex

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004584

C

Seeded From UniProt

GO:0030870

Mre11 complex

GO_REF:0000024

ISO: Inferred from Sequence Orthology

SGD:S000005194

C

Seeded From UniProt

GO:0030870

Mre11 complex

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

C

Seeded From UniProt

GO:0031573

intra-S DNA damage checkpoint

PMID:12944482[4]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0032508

DNA duplex unwinding

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

P

Seeded From UniProt

GO:0035861

site of double-strand break

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

C

Seeded From UniProt

GO:0042138

meiotic DNA double-strand break formation

PMID:12019258[5]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0042138

meiotic DNA double-strand break formation

PMID:15654094[6]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:0043047

single-stranded telomeric DNA binding

GO_REF:0000024

ISO: Inferred from Sequence Orthology

SGD:S000005194

F

Seeded From UniProt

GO:0043047

single-stranded telomeric DNA binding

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

GO:0046872

metal ion binding

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0479

F

Seeded From UniProt

GO:0046939

nucleotide phosphorylation

GO_REF:0000108

IEA: Inferred from Electronic Annotation

GO:0004017

P

Seeded From UniProt

GO:0051321

meiotic cell cycle

GO_REF:0000037

IEA: Inferred from Electronic Annotation

UniProtKB-KW:KW-0469

P

Seeded From UniProt

GO:0051880

G-quadruplex DNA binding

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

GO:0070192

chromosome organization involved in meiotic cell cycle

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

P

Seeded From UniProt

GO:1990426

mitotic recombination-dependent replication fork processing

PMID:23093942[7]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

GO:1990918

double-strand break repair involved in meiotic recombination

PMID:15238514[8]

IMP: Inferred from Mutant Phenotype

-

P

Seeded From UniProt

contributes_to

GO:0000014

single-stranded DNA endodeoxyribonuclease activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

contributes_to

GO:0004003

ATP-dependent DNA helicase activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

contributes_to

GO:0008408

3'-5' exonuclease activity

GO_REF:0000033

IBA: Inferred from Biological aspect of Ancestor

PANTHER:PTN000429848

F

Seeded From UniProt

GO:0006303

double-strand break repair via nonhomologous end joining

PMID:28292918[9]

IMP: Inferred from Mutant Phenotype

P

Rad50 is part of the Mre11-Rad50-Nbs1 complex which plays a role in DNA repair processes including DNA damage checkpoint and recruitment of telomerase. Rad50 is responsible for stimulating ATP dependent DNA unwinding. Fig. 4. Efficient NHEJ requires Ku70, DNA ligase 4 and MRN. (Mre11-Rad50-Nbs1=MRN)

complete
CACAO 12784

Notes

References

See Help:References for how to manage references in GONUTS.

  1. Subramanian, L et al. (2008) Recombination-based telomere maintenance is dependent on Tel1-MRN and Rap1 and inhibited by telomerase, Taz1, and Ku in fission yeast. Mol. Cell. Biol. 28 1443-55 PubMed GONUTS page
  2. Tomita, K et al. (2003) Competition between the Rad50 complex and the Ku heterodimer reveals a role for Exo1 in processing double-strand breaks but not telomeres. Mol. Cell. Biol. 23 5186-97 PubMed GONUTS page
  3. Hartsuiker, E et al. (2001) Fission yeast Rad50 stimulates sister chromatid recombination and links cohesion with repair. EMBO J. 20 6660-71 PubMed GONUTS page
  4. Chahwan, C et al. (2003) The fission yeast Rad32 (Mre11)-Rad50-Nbs1 complex is required for the S-phase DNA damage checkpoint. Mol. Cell. Biol. 23 6564-73 PubMed GONUTS page
  5. Farah, JA et al. (2002) A 160-bp palindrome is a Rad50.Rad32-dependent mitotic recombination hotspot in Schizosaccharomyces pombe. Genetics 161 461-8 PubMed GONUTS page
  6. Farah, JA et al. (2005) A novel recombination pathway initiated by the Mre11/Rad50/Nbs1 complex eliminates palindromes during meiosis in Schizosaccharomyces pombe. Genetics 169 1261-74 PubMed GONUTS page
  7. Iraqui, I et al. (2012) Recovery of arrested replication forks by homologous recombination is error-prone. PLoS Genet. 8 e1002976 PubMed GONUTS page
  8. Young, JA et al. (2004) Conserved and nonconserved proteins for meiotic DNA breakage and repair in yeasts. Genetics 167 593-605 PubMed GONUTS page
  9. Li, Y et al. (2017) Non-Homologous End-Joining with Minimal Sequence Loss Is Promoted by the Mre11-Rad50-Nbs1-Ctp1 Complex in Schizosaccharomyces pombe. Genetics PubMed GONUTS page