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This is your chance to make annotations OR challenge other team's annotations. You may also DEFEND or suggest improvements to your own annotations IF they have been challenged. Please note, although we ENCOURAGE challenges, an excess of identical challenges that do not appear to be applicable to the annotation or well thought out will be considered spam and ignored.
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SCHPO:DCR1
Contents
Species (Taxon ID) | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast). (284812) | |
Gene Name(s) | dcr1 | |
Protein Name(s) | Protein Dicer
Cell cycle control protein dcr1 RNA interference pathway protein dcr1 Endoribonuclease dcr1 ATP-dependent helicase dcr1 | |
External Links | ||
UniProt | Q09884 | |
EMBL | CU329672 | |
PIR | T39130 | |
RefSeq | NP_588215.2 | |
PDB | 2L6M | |
PDBsum | 2L6M | |
ProteinModelPortal | Q09884 | |
STRING | Q09884 | |
EnsemblFungi | SPCC188.13c.1 | |
GeneID | 2538930 | |
GenomeReviews | CU329672_GR | |
KEGG | spo:SPCC188.13c | |
PomBase | SPCC188.13c | |
eggNOG | COG1111 | |
KO | K11592 | |
OMA | RMCLICN | |
OrthoDB | EOG4PRWZS | |
NextBio | 20800107 | |
GO | GO:0005829 GO:0005720 GO:0005524 GO:0003677 GO:0004386 GO:0046872 GO:0004540 GO:0004525 GO:0003723 GO:0007049 GO:0034613 GO:0031048 GO:0007059 GO:0035389 GO:0030422 GO:0090052 GO:0051983 GO:0051570 | |
Gene3D | 1.10.1520.10 | |
InterPro | IPR005034 IPR006935 IPR014001 IPR001650 IPR000999 | |
Pfam | PF03368 PF00271 PF04851 PF00636 | |
SMART | SM00487 SM00490 SM00535 | |
SUPFAM | SSF69065 | |
PROSITE | PS51327 PS51192 PS51194 PS00517 PS50142 |
Annotations
Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
---|---|---|---|---|---|---|---|---|
GO:0000166 |
nucleotide binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0003676 |
nucleic acid binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0003677 |
DNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0003723 |
RNA binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004386 |
helicase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004386 |
helicase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004518 |
nuclease activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004519 |
endonuclease activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004525 |
ribonuclease III activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0004540 |
ribonuclease activity |
IDA: Inferred from Direct Assay |
- |
F |
Seeded From UniProt |
|||
GO:0005524 |
ATP binding |
IC: Inferred by Curator |
GO:0008026 |
F |
Seeded From UniProt |
|||
GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0005524 |
ATP binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
GO:0005634 |
nucleus |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
GO:0005720 |
nuclear heterochromatin |
IC: Inferred by Curator |
GO:0016442 |
C |
Seeded From UniProt |
|||
GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
GO:0005737 |
cytoplasm |
IEA: Inferred from Electronic Annotation |
C |
Seeded From UniProt |
||||
GO:0005737 |
cytoplasm |
IDA: Inferred from Direct Assay |
- |
C |
Seeded From UniProt |
|||
GO:0005829 |
cytosol |
IDA: Inferred from Direct Assay |
- |
C |
Seeded From UniProt |
|||
GO:0006396 |
RNA processing |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
GO:0006396 |
RNA processing |
IDA: Inferred from Direct Assay |
- |
P |
Seeded From UniProt |
|||
GO:0007049 |
cell cycle |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
GO:0007059 |
chromosome segregation |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0016787 |
hydrolase activity |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0016891 |
endoribonuclease activity, producing 5'-phosphomonoesters |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0030422 |
production of siRNA involved in RNA interference |
IDA: Inferred from Direct Assay |
- |
P |
Seeded From UniProt |
|||
GO:0031047 |
gene silencing by RNA |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
GO:0031048 |
chromatin silencing by small RNA |
TAS: Traceable Author Statement |
- |
P |
Seeded From UniProt |
|||
GO:0034613 |
cellular protein localization |
IMP: Inferred from Mutant Phenotype |
- |
P |
Seeded From UniProt |
|||
GO:0035389 |
establishment of chromatin silencing at silent mating-type cassette |
IMP: Inferred from Mutant Phenotype |
- |
P |
Seeded From UniProt |
|||
GO:0046872 |
metal ion binding |
IEA: Inferred from Electronic Annotation |
F |
Seeded From UniProt |
||||
GO:0051570 |
regulation of histone H3-K9 methylation |
IMP: Inferred from Mutant Phenotype |
- |
P |
Seeded From UniProt |
|||
GO:0051983 |
regulation of chromosome segregation |
IMP: Inferred from Mutant Phenotype |
- |
P |
Seeded From UniProt |
|||
GO:0090052 |
regulation of chromatin silencing at centromere |
IMP: Inferred from Mutant Phenotype |
- |
P |
Seeded From UniProt |
|||
GO:0090305 |
nucleic acid phosphodiester bond hydrolysis |
IEA: Inferred from Electronic Annotation |
P |
Seeded From UniProt |
||||
GO:0010629 |
negative regulation of gene expression |
IMP: Inferred from Mutant Phenotype |
P |
Figure 4. Shows the relative protein expression level is increased in the Dcr1 knockout strain when compared to the wild-type strain. Lower protein expression is indicative of lower gene expression. |
complete | |||
edit table |
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.0 1.1 1.2 Qian, Z et al. (2005) Expression and purification of the carboxyl terminus domain of Schizosaccharomyces pombe dicer in Escherichia coli. Protein Pept. Lett. 12 311-4 PubMed GONUTS page
- ↑ 2.0 2.1 Matsuyama, A et al. (2006) ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe. Nat. Biotechnol. 24 841-7 PubMed GONUTS page
- ↑ Motamedi, MR et al. (2004) Two RNAi complexes, RITS and RDRC, physically interact and localize to noncoding centromeric RNAs. Cell 119 789-802 PubMed GONUTS page
- ↑ Sadaie, M et al. (2004) A chromodomain protein, Chp1, is required for the establishment of heterochromatin in fission yeast. EMBO J. 23 3825-35 PubMed GONUTS page
- ↑ Carmichael, JB et al. (2004) ago1 and dcr1, two core components of the RNA interference pathway, functionally diverge from rdp1 in regulating cell cycle events in Schizosaccharomyces pombe. Mol. Biol. Cell 15 1425-35 PubMed GONUTS page
- ↑ 6.0 6.1 Volpe, TA et al. (2002) Regulation of heterochromatic silencing and histone H3 lysine-9 methylation by RNAi. Science 297 1833-7 PubMed GONUTS page
- ↑ Provost, P et al. (2002) Dicer is required for chromosome segregation and gene silencing in fission yeast cells. Proc. Natl. Acad. Sci. U.S.A. 99 16648-53 PubMed GONUTS page
- ↑ Gobeil, LA et al. (2008) Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe. Front. Biosci. 13 2203-15 PubMed GONUTS page
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- GO:0051570 ! regulation of histone H3-K9 methylation
- GO:0090052 ! regulation of chromatin silencing at centromere
- GO:0051983 ! regulation of chromosome segregation
- GO:0004525 ! ribonuclease III activity
- GO:0004540 ! ribonuclease activity
- GO:0016442 ! RISC complex
- GO:0003723 ! RNA binding
- GO:0006396 ! RNA processing