PMID:17981703

Citation	Gobeil, LA, Plante, P, Rohani, M, Ouellette, M and Provost, P (2008) Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe. Front. Biosci. 13:2203-15
Abstract	The ribonuclease III Dicer (Dcr1) has been shown to be required for chromosome segregation and gene silencing in Schizosaccharomyces pombe. These effects are thought to be transcriptional, mediated by formation and maintenance of heterochromatin, and guided by small RNAs derived from Dcr1 along a process known as RNA interference. In order to get further insights into the gene regulatory role of Dcr1, we performed comparative analyses of dcr1 knockout and wild-type fission yeast strains. Analysis of part of the soluble proteomes identified eight cellular proteins whose expression is under Dcr1 control, three of which are integral constituents of the glycolysis pathway. Further correlations with their respective mRNA transcript levels are compatible with the existence of a post-transcriptional gene regulatory mechanism involving Dcr1 or a Dcr1 complex. Experiments designed to identify components of Dcr1 complexes unveiled two novel Dcr1 interactors, namely the zinc finger protein Byr3 and the ribosomal protein L12. Consistently enriched in Dcr1 immune complexes, Byr3 and L12 may link Dcr1 to the transcriptional and translational machineries, respectively, and contribute to post-transcriptional gene regulation in fission yeast.
Links	PubMed PMC2930013
Keywords	Electrophoresis, Gel, Two-Dimensional; Gene Expression Regulation, Fungal; Heat-Shock Proteins/physiology; Immunoprecipitation; Intracellular Signaling Peptides and Proteins; RNA Processing, Post-Transcriptional; RNA, Messenger/metabolism; Recombination, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III/genetics; Ribonuclease III/physiology; Ribosomes/metabolism; Schizosaccharomyces/genetics; Schizosaccharomyces/physiology; Schizosaccharomyces pombe Proteins/genetics; Schizosaccharomyces pombe Proteins/physiology; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transcription Factors/genetics; Transcription Factors/physiology; Zinc Fingers
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SCHPO:DCR1	involved_in	GO:0010629: negative regulation of gene expression	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete
SCHPO:DCR1		GO:0010629: negative regulation of gene expression	ECO:0000315:		P		Figure 4. Shows the relative protein expression level is increased in the Dcr1 knockout strain when compared to the wild-type strain. Lower protein expression is indicative of lower gene expression.	complete CACAO 7017

References

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