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PMID:30326905
| Citation |
Sviben, D, Forcic, D, Halassy, B, Allmaier, G, Marchetti-Deschmann, M and Brgles, M (2018) Mass spectrometry-based investigation of measles and mumps virus proteome. Virol. J. 15:160 |
|---|---|
| Abstract |
Measles (MEV) and mumps virus (MUV) are enveloped, non-segmented, negative single stranded RNA viruses of the family Paramyxoviridae, and are the cause of measles and mumps, respectively, both preventable by vaccination. Aside from proteins coded by the viral genome, viruses are considered to contain host cell proteins (HCPs). The presence of extracellular vesicles (ECVs), which are often co-purified with viruses due to their similarity in size, density and composition, also contributes to HCPs detected in virus preparations, and this has often been neglected. The aim was to identify which virus-coded proteins are present in MEV and MUV virions, and to try to detect which HCPs, if any, are incorporated inside the virions or adsorbed on their outer surface, and which are more likely to be a contamination from co-purified ECVs. |
| Links |
PubMed PMC6192076 Online version:10.1186/s12985-018-1073-9 |
| Keywords |
Animals; Cercopithecus aethiops; Hydrophobic and Hydrophilic Interactions; Measles/virology; Measles virus/chemistry; Mumps/virology; Mumps virus/chemistry; Proteome/analysis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Vero Cells; Viral Proteins/analysis; Virion/chemistry |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein M (matrix protein) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. This Matrix protein is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q6RXY8 (Q6RXY8_MEASZ), Submitted name: Matrix protein Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 2 shows that protein M (matrix protein) is a viral protein of the Mumps virus as determined through MALDI-TOF/TOF-mass spectrometry. Protein M is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MUV genome with the UniProt entry. UniProtKB - Q5SC54 (Q5SC54_9MONO) Submitted name: M protein Organism: Mumps virus strain L-Zagreb |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein H (hemagglutinin) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. Hemagglutinin is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q9IC33 (Q9IC33_MEASZ), Submitted name: Hemagglutinin Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein N (nucleoprotein) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. Nucleoprotein is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q89933 (NCAP_MEASF), nucleoprotein Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein F (Fusion glycoprotein F0) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. Fusion glycoprotein F0 is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - O93055 (O93055_MEASZ), Fusion glycoprotein F0 Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein P (phosphoprotein) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. Phosphoprotein is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q77M25 (Q77M25_MEASZ), Submitted name: Phosphoprotein Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1 shows that protein L (RNA-directed RNA polymerase L) is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. RNA-directed RNA polymerase L is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q77M22 (Q77M22_MEASZ), RNA-directed RNA polymerase L Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 1A, 1B and 1C all show that protein C is a viral protein of the Measles virus as determined through MALDI-TOF/TOF-mass spectrometry. Protein C is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MEV genome with the UniProt entry. UniProtKB - Q77M23 (Q77M23_MEASZ), Submitted name: C protein Organism: Measles virus (strain Edmonston-Zagreb vaccine) (MeV) (Subacute sclerose panencephalitis virus) |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 2 shows that protein L (RNA-directed RNA polymerase L) is a viral protein of the Mumps virus as determined through MALDI-TOF/TOF-mass spectrometry. RNA-directed RNA polymerase L is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MUV genome with the UniProt entry. UniProtKB - Q5SC50 (Q5SC50_9MONO), RNA-directed RNA polymerase L Organism: Mumps virus strain L-Zagreb |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 2 shows that protein HN is a viral protein of the Mumps virus as determined through MALDI-TOF/TOF-mass spectrometry. Protein HN is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MUV genome with the UniProt entry. UniProtKB - Q5SC51 (Q5SC51_9MONO), Submitted name: HN Organism: Mumps virus strain L-Zagreb |
complete | ||||
| GO:0019012: virion |
ECO:0005527: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion |
C |
Figure 2 shows that protein NP (nucleocapsid) is a viral protein of the Mumps virus as determined through MALDI-TOF/TOF-mass spectrometry. Protein NP is displayed on an SDS-PAGE purified by UC after MALDI-TOF/TOF MS analysis. Table S1 includes proteins coded by MUV genome with the UniProt entry. UniProtKB - Q5SC49 (Q5SC49_9MONO), Nucleocapsid Organism: Mumps virus strain L-Zagreb |
complete | ||||
Notes
See also
References
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