PMID:11226670

Citation	Wyneken, U, Smalla, KH, Marengo, JJ, Soto, D, de la Cerda, A, Tischmeyer, W, Grimm, R, Boeckers, TM, Wolf, G, Orrego, F and Gundelfinger, ED (2001) Kainate-induced seizures alter protein composition and N-methyl-D-aspartate receptor function of rat forebrain postsynaptic densities. Neuroscience 102:65-74
Abstract	The postsynaptic density is a highly dynamic structure, which is reorganized in an activity-dependent manner. An animal model for temporal lobe epilepsy, i.e. kainate-induced limbic seizures in rats, was used to study changes in postsynaptic density composition after extensive synaptic activity. Six hours after kainate injection, the protein content of the postsynaptic density fractions from rats that developed strong seizures was increased three-fold compared to saline-treated controls. Immunoblot analysis revealed that the relative amounts of metabotropic glutamate receptor 1alpha, N-ethylmaleimide-sensitive fusion protein, protein kinases C, Fyn and TrkB, as well as the neuronal nitric oxide synthase, were significantly higher in seizure-developing than in control rats. In contrast, the relative contents of the kainate receptor KA2 subunit, beta-actin, alpha-adducin and the membrane-associated guanylate kinase homolog SAP90/PSD-95 were decreased. The relative amounts of additional postsynaptic density proteins, including alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate and N-methyl-D-aspartate receptor subunits, calcium/calmodulin-dependent kinase type II, casein kinase 2, tubulin, microtubule-associated protein 2B, the membrane-associated guanylate kinase homolog SAP102, and proline-rich synapse-associated protein 1/cortactin binding protein 1/Shank2 remained essentially unchanged. To assess possible changes in postsynaptic performance, postsynaptic densities were isolated from control and epileptic rats, incorporated into giant liposomes and N-methyl-D-aspartate receptor currents were recorded. A significant reduction in the mean conductance was observed in patches containing postsynaptic densities from animals with high seizure activity. This was due to the presence of reduced conductance levels in each membrane patch compared to control postsynaptic density preparations. From these data, we suggest that intense synaptic activity associated with seizures modifies the composition of postsynaptic densities and has profound consequences on the function of the N-methyl-D-aspartate receptors present in them. This rearrangement may accompany impairment of synaptic plasticity.
Links	PubMed
Keywords	Animals; Cytoskeleton/metabolism; Disease Models, Animal; Epilepsy, Temporal Lobe/metabolism; Epilepsy, Temporal Lobe/physiopathology; Excitatory Amino Acid Agonists/pharmacology; Kainic Acid/pharmacology; Male; Nerve Tissue Proteins/drug effects; Nerve Tissue Proteins/metabolism; Neurons/drug effects; Neurons/metabolism; Phosphorylation; Prosencephalon/drug effects; Prosencephalon/metabolism; Prosencephalon/physiopathology; Rats; Rats, Wistar; Receptors, Kainic Acid/metabolism; Receptors, Metabotropic Glutamate/metabolism; Receptors, N-Methyl-D-Aspartate/drug effects; Receptors, N-Methyl-D-Aspartate/metabolism; Seizures/chemically induced; Seizures/metabolism; Seizures/physiopathology; Subcellular Fractions/metabolism; Synaptic Membranes/drug effects; Synaptic Membranes/metabolism; Tyrosine/metabolism
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
RAT:DLG4	part_of	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:NOS1	part_of	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete
RAT:GRM1	part_of	GO:0014069: postsynaptic density	ECO:0000314: direct assay evidence used in manual assertion		C		Seeded From UniProt	complete

References

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