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MOUSE:GLIS2
Contents
| Species (Taxon ID) | Mus musculus (Mouse). (10090) | |
| Gene Name(s) | Glis2 (synonyms: Gli5, Nkl) | |
| Protein Name(s) | Zinc finger protein GLIS2
GLI-similar 2 Neuronal Krueppel-like protein Zinc finger protein GLI5 | |
| External Links | ||
| UniProt | Q8VDL9 | |
| EMBL | AF249340 AF325913 AF336135 BC021517 | |
| CCDS | CCDS27920.1 | |
| RefSeq | NP_112461.2 XP_006522831.1 | |
| UniGene | Mm.134072 | |
| ProteinModelPortal | Q8VDL9 | |
| SMR | Q8VDL9 | |
| BioGrid | 219913 | |
| PhosphoSite | Q8VDL9 | |
| PRIDE | Q8VDL9 | |
| Ensembl | ENSMUST00000014447 | |
| GeneID | 83396 | |
| KEGG | mmu:83396 | |
| UCSC | uc007xzw.1 | |
| CTD | 84662 | |
| MGI | MGI:1932535 | |
| eggNOG | COG5048 | |
| GeneTree | ENSGT00760000118771 | |
| HOGENOM | HOG000065778 | |
| HOVERGEN | HBG101807 | |
| InParanoid | Q8VDL9 | |
| KO | K09233 | |
| OMA | DKCLSPE | |
| OrthoDB | EOG70CR6P | |
| PhylomeDB | Q8VDL9 | |
| TreeFam | TF351425 | |
| NextBio | 350519 | |
| PRO | PR:Q8VDL9 | |
| Proteomes | UP000000589 | |
| Bgee | Q8VDL9 | |
| CleanEx | MM_GLIS2 | |
| ExpressionAtlas | Q8VDL9 | |
| Genevestigator | Q8VDL9 | |
| GO | GO:0005737 GO:0016607 GO:0005634 GO:0005667 GO:0003677 GO:0046872 GO:0001077 GO:0003700 GO:0044212 GO:0030154 GO:0043433 GO:0045879 GO:0000122 GO:0045892 GO:0007399 GO:0045944 GO:0045893 GO:0006357 | |
| Gene3D | 3.30.160.60 | |
| InterPro | IPR007087 IPR015880 IPR013087 | |
| Pfam | PF00096 | |
| SMART | SM00355 | |
| PROSITE | PS00028 PS50157 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
| GO:0008139 |
nuclear localization sequence binding |
ECO:0000314 |
F |
Figure 1: Subcellular localisation experiments have shown that the 237-246 region of the Glis2 protein is essential for the nuclear localisation to the nucleus. |
complete | |||||
| GO:0010843 |
promoter binding |
ECO:0000314 |
F |
Figure 2: EMSA experiments have shown that Glis2 binds to the GlisBS. Figure 2A shows that unlabelled GlisBS competed more efficiently than the labelled GlisBS. Figure 2C shows that when unlabelled Glis2 mutants were added to the EMSA, they did not compete efficiently with the labelled GlisBS for Gli2 binding. This data suggests that Glis2 binds to the GlisBS region of the promoter. |
complete | |||||
| GO:0016563 |
transcription activator activity |
ECO:0000315 |
F |
Figure 4: Using Luciferase assay experiments, it was shown that Glis2 mutants did not activate the Ins2 promoter, whereas the wild-type Glis2 activated the promoter. Also, when regions of the C and N terminus were deleted, there was a decrease of activation compared to the full length. |
complete | |||||
| GO:0045893 |
positive regulation of transcription, DNA-dependent |
ECO:0000315 |
P |
Figure 2: EMSA experiments show that Glis2 binds to a specific DNA sequence (GlisBS). Figure 5C: EMSA experiments show that Glis2 binds directly to GlisBS (-99 and -263 regions). Figure 6: minimal activation of Ins2 occurs when deletions and point mutations are added to the -99 and -263 regions of the GlisBS promoter of Ins2. |
complete | |||||
| GO:0005634 |
nucleus |
ECO:0000314 |
C |
Figure 1: Confocal micrographs show the nuclear localisation of the Glis2 transcription factor |
complete | |||||
| GO:0065004 |
protein-DNA complex assembly |
ECO:0000314 |
P |
Figure 2 and 4: EMSA experiments show that Glis2 protein binds to the GlisBS sequence of the DNA promoter. Glis2 mutants do not bind as efficiently, which suggests that this sequence binding is important |
complete | |||||
| GO:0000981 |
sequence-specific DNA binding RNA polymerase II transcription factor activity |
ECO:0000315 |
F |
Figure 2: EMSA experiments have shown that Glis2 binds to GlisBS region efficiently. Figure 4: EMSA experiments have shows that the activation of the Ins2 promoter is through the competition binding of the GlisBS region of the Ins2 promoter. |
complete | |||||
| GO:0043565 |
sequence-specific DNA binding |
ECO:0000315 |
F |
Figure 2, 4: These EMSA experiments have shown through competition experiments have Glis2 binds efficiently to GlisBS region. However, when GlisBS mutants were used, they did not bind to them. Figure 6: Deletions and point mutations in the Ins2 promoter using luciferase assay shows that Glis2 binds specifically to the GlisBS promoter region of Ins2. |
complete | |||||
| GO:0043433 |
negative regulation of transcription factor activity |
ECO:0000314 |
P |
Figure 3: Luciferase assays have shown that the presence of the Glis2 protein supresses the activation of the GlisBS region by the Gli1 transcription factor. Figure 8: Luciferase assays for mutants in the Glis2 protein reduced the repression of Gli1 activation of GlisBS. |
complete | |||||
|
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0044212 |
transcription regulatory region DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0043433 |
negative regulation of DNA-binding transcription factor activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0001228 |
DNA-binding transcription activator activity, RNA polymerase II-specific |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
occurs_at:(SO:0001952) |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045893 |
positive regulation of transcription, DNA-templated |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045892 |
negative regulation of transcription, DNA-templated |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045879 |
negative regulation of smoothened signaling pathway |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016607 |
nuclear speck |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0045944 |
positive regulation of transcription by RNA polymerase II |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:1932535 |
P |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0007417 |
central nervous system development |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
MGI:MGI:106679 |
P |
Seeded From UniProt |
complete | ||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0003002 |
C |
Seeded From UniProt |
complete | ||
|
enables |
GO:0000981 |
DNA-binding transcription factor activity, RNA polymerase II-specific |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0003002 |
F |
Seeded From UniProt |
complete | ||
|
enables |
GO:0000977 |
RNA polymerase II regulatory region sequence-specific DNA binding |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0003002 |
F |
Seeded From UniProt |
complete | ||
|
involved_in |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
ECO:0000318 |
biological aspect of ancestor evidence used in manual assertion |
FB:FBgn0033782 |
P |
Seeded From UniProt |
complete | ||
|
acts_upstream_of_or_within |
GO:1900182 |
positive regulation of protein localization to nucleus |
ECO:0000316 |
genetic interaction evidence used in manual assertion |
MGI:MGI:98397 |
P |
has_direct_input:(UniProtKB:P30999) |
Seeded From UniProt |
complete | |
|
part_of |
GO:0097730 |
non-motile cilium |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0061005 |
cell differentiation involved in kidney development |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
MGI:MGI:3719836 |
P |
occurs_in:(EMAPA:17373) |
Seeded From UniProt |
complete | |
|
acts_upstream_of_or_within |
GO:0060994 |
regulation of transcription from RNA polymerase II promoter involved in kidney development |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
MGI:MGI:3785519 |
P |
occurs_in:(EMAPA:17373)|occurs_in:(EMAPA:17373) |
Seeded From UniProt |
complete | |
|
acts_upstream_of_or_within |
GO:0006357 |
regulation of transcription by RNA polymerase II |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
P |
Seeded From UniProt |
Missing: with/from | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005667 |
transcription factor complex |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
C |
Seeded From UniProt |
Missing: with/from | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000314 |
direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003700 |
DNA-binding transcription factor activity |
ECO:0000250 |
sequence similarity evidence used in manual assertion |
F |
Seeded From UniProt |
Missing: with/from | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0001822 |
kidney development |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
MGI:MGI:3785519 |
P |
occurs_in:(EMAPA:17373) |
Seeded From UniProt |
complete | |
|
enables |
GO:0000977 |
RNA polymerase II regulatory region sequence-specific DNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
acts_upstream_of_or_within |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
ECO:0000315 |
mutant phenotype evidence used in manual assertion |
MGI:MGI:3719836 |
P |
occurs_in:(EMAPA:17373) |
Seeded From UniProt |
complete | |
|
acts_upstream_of_or_within |
GO:0000122 |
negative regulation of transcription by RNA polymerase II |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
has_regulation_target:(MGI:MGI:95727) |
Seeded From UniProt |
complete | ||
|
enables |
GO:0003676 |
nucleic acid binding |
ECO:0000256 |
match to sequence model evidence used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0030154 |
cell differentiation |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007275 |
multicellular organism development |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005737 |
cytoplasm |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0005634 |
nucleus |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0007399 |
nervous system development |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0046872 |
metal ion binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0016607 |
nuclear speck |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
C |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 1.14 Vasanth, S et al. (2011) Identification of nuclear localization, DNA binding, and transactivating mechanisms of Kruppel-like zinc finger protein Gli-similar 2 (Glis2). J. Biol. Chem. 286 4749-59 PubMed GONUTS page
- ↑ 2.0 2.1 2.2 Zhang, F et al. (2002) Characterization of Glis2, a novel gene encoding a Gli-related, Krüppel-like transcription factor with transactivation and repressor functions. Roles in kidney development and neurogenesis. J. Biol. Chem. 277 10139-49 PubMed GONUTS page
- ↑ Li, B et al. (2011) Increased hedgehog signaling in postnatal kidney results in aberrant activation of nephron developmental programs. Hum. Mol. Genet. 20 4155-66 PubMed GONUTS page
- ↑ 4.0 4.1 4.2 4.3 4.4 4.5 Gaudet, P et al. (2011) Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium. Brief. Bioinformatics 12 449-62 PubMed GONUTS page
- ↑ 5.0 5.1 5.2 5.3 Hosking, CR et al. (2007) The transcriptional repressor Glis2 is a novel binding partner for p120 catenin. Mol. Biol. Cell 18 1918-27 PubMed GONUTS page
- ↑ 6.0 6.1 6.2 6.3 Attanasio, M et al. (2007) Loss of GLIS2 causes nephronophthisis in humans and mice by increased apoptosis and fibrosis. Nat. Genet. 39 1018-24 PubMed GONUTS page
- ↑ 7.0 7.1 7.2 Kim, YS et al. (2008) Kruppel-like zinc finger protein Glis2 is essential for the maintenance of normal renal functions. Mol. Cell. Biol. 28 2358-67 PubMed GONUTS page
- ↑ 8.0 8.1 8.2 8.3 Lamar, E et al. (2001) Identification of NKL, a novel Gli-Kruppel zinc-finger protein that promotes neuronal differentiation. Development 128 1335-46 PubMed GONUTS page
- ↑ Sakai-Kato, K et al. () CD spectra show the relational style between Zic-, Gli-, Glis-zinc finger protein and DNA. Biochim. Biophys. Acta 1784 1011-9 PubMed GONUTS page
c
d
g
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- GO:0045879 ! negative regulation of smoothened signaling pathway
- GO:0043433 ! negative regulation of DNA-binding transcription factor activity
- GO:0000122 ! negative regulation of transcription by RNA polymerase II
- GO:0045892 ! negative regulation of DNA-templated transcription
- GO:0007399 ! nervous system development
- GO:0097730 ! non-motile cilium
- GO:0008139 ! nuclear localization sequence binding
- GO:0016607 ! nuclear speck
- GO:0003676 ! nucleic acid binding
- GO:0005634 ! nucleus
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