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Category:Team Lysis to Kill

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StatusPageUserDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
unacceptableLIBAP:C6XGA9Baileybechtold, Team Lysis to Kill2017-04-07 10:34:04 CDTGO:0008236 serine-type peptidase activity (F)PMID:28066334ECO:0000315 mutant phenotype evidence used in manual assertion

Liberibacter asiaticus lepB could partially complement the lepB mutant of E.coli. lepB is the gene that encodes Serine peptidase I.

Figure 4 shows that the wild type E. coli grew at 37 degrees celcius and 42. The amber E. coli mutant grew at 37 but not 42. The lepB homolog of Liberibacter asiaticus partially restores growth of the mutant strain to result in growth at 37 and 42.

challenge
acceptableLIBAP:C6XGA9Baileybechtold, Team Lysis to Kill2017-04-07 10:19:02 CDTGO:0008236 serine-type peptidase activity (F)PMID:28066334ECO:0000247 sequence alignment evidence used in manual assertion

Experimenters conducted a sequence alignment with Liberibacter asiaticus (strain 62) with Escherichia coli (strain K-12) to confirm that serine-type pepsidase I (SPase I) is conserved. The sequence alignment was also comparing Liberibacter asiaticus (strain:gxpsy) and Liberibacter asiaticus (strain:Ishi-1).

Suplementary Figure 1 shows the identity for the SPase I of the three Liberibacter asiaticus strains is 100%. The Liberibacter asiaticus SPase I protein shares 34% identity and 52% similarity with that of E. coli.

challenge
unacceptableLIBAP:C6XHE2Shelbylmalmanger, Team Lysis to Kill2017-04-07 10:16:27 CDTGO:0051920 peroxiredoxin activity (F)PMID:27987036ECO:0000247 sequence alignment evidence used in manual assertion

Candidatus LIberibacter aciaticus, CLa

Bacterioferritin comigratory protein, BCP

In figure 1 they did a sequence alignment for Cla-BCP with Brucella melitensis strain 16M.

challenge
unacceptableDIACI:A0A0U4DX43Baileybechtold, Team Lysis to Kill2017-04-06 20:20:29 CDTGO:0004197 cysteine-type endopeptidase activity (F)PMID:26717484ECO:0000247 sequence alignment evidence used in manual assertion

In the paper, the gene sequence of Diaphorina citri is compared in a sequence analysis with Nilaparvata lugens, Aphis citricidus, Riportus pedestris, and Acyrthosiphon pisum.

Figure 1 shows this sequence alignment. The other bacteria in the alignment display similar cathepsin B-like cysteine peptidase activity from hemipterans. The dashed boxes in Figure 1 display a probable occlusion loop characteristic of cathepsin B-like cysteine peptidases.

challenge
unacceptableLIBAP:C6XHE2Shelbylmalmanger, Team Lysis to Kill2017-04-05 15:30:12 CDTGO:0051920 peroxiredoxin activity (F)PMID:27987036ECO:0000247 sequence alignment evidence used in manual assertion

Candidatus LIberibacter aciaticus, Cla

Bacterioferritin comigratory protein, BCP

In figure 1 they did a sequence alignment for Cla-BCP with Helicobacter pylori strain J99.

challenge
unacceptableLIBAP:C6XFZ2Jacobheiling, Team Lysis to Kill2017-03-24 11:18:25 CDTGO:0042964 thioredoxin reduction (P)PMID:27987036ECO:0000314 direct assay evidence used in manual assertion

Thioredoxin activity also observed in PMID:20335172, their results were not shown in that experiment but were mentioned.

challenge
updatedbyinstructorLIBAP:C6XHE2Sarahswartz, Team Lysis to Kill2017-03-23 16:35:29 CDTGO:0042262 DNA protection (P)PMID:27987036ECO:0000314 direct assay evidence used in manual assertion

Figure 8a shows the clear protective nature of CLa-BCP on coiled DNA. Lane 1 was the control group for demonstrating protected (coiled) DNA. Lanes 3 and 4 which contained CLa-BCP show nicked circular (NC) DNA and supercoiled (SC) DNA rather than in lane 2 which showed uncoiled, unprotected, linear DNA from the effects of H2O2.

"The BCP gene (GenBank: ACT56685.1, originally annotated as BCP gene) was amplified by PCR using CLA genomic DNA." This corresponds to CLIBASIA_00485.

challenge
updatedbyinstructorLIBAP:C6XHE2Sarahswartz, Team Lysis to Kill2017-03-23 15:54:49 CDTGO:0051920 peroxiredoxin activity (F)PMID:27987036ECO:0000314 direct assay evidence used in manual assertion

Figure 3, part a, shows the peroxiredoxin assay and the significance of CLa-BCP in removing H2O2. There is an indirect relationship between the amount of Cla-BCP and the percentage of disappearance of H2O2 Liberibacter asiaticus" (aka Candidatus Liberibacter asiaticus psy62)

challenge

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Pages in category "Team Lysis to Kill"

The following 4 pages are in this category, out of 4 total.


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