Category:Team Lysis to Kill

Liberibacter asiaticus lepB could partially complement the lepB mutant of E.coli. lepB is the gene that encodes Serine peptidase I.

Figure 4 shows that the wild type E. coli grew at 37 degrees celcius and 42. The amber E. coli mutant grew at 37 but not 42. The lepB homolog of Liberibacter asiaticus partially restores growth of the mutant strain to result in growth at 37 and 42.

Experimenters conducted a sequence alignment with Liberibacter asiaticus (strain 62) with Escherichia coli (strain K-12) to confirm that serine-type pepsidase I (SPase I) is conserved. The sequence alignment was also comparing Liberibacter asiaticus (strain:gxpsy) and Liberibacter asiaticus (strain:Ishi-1).

Suplementary Figure 1 shows the identity for the SPase I of the three Liberibacter asiaticus strains is 100%. The Liberibacter asiaticus SPase I protein shares 34% identity and 52% similarity with that of E. coli.

Candidatus LIberibacter aciaticus, CLa

Bacterioferritin comigratory protein, BCP

In figure 1 they did a sequence alignment for Cla-BCP with Brucella melitensis strain 16M.

In the paper, the gene sequence of Diaphorina citri is compared in a sequence analysis with Nilaparvata lugens, Aphis citricidus, Riportus pedestris, and Acyrthosiphon pisum.

Figure 1 shows this sequence alignment. The other bacteria in the alignment display similar cathepsin B-like cysteine peptidase activity from hemipterans. The dashed boxes in Figure 1 display a probable occlusion loop characteristic of cathepsin B-like cysteine peptidases.

Candidatus LIberibacter aciaticus, Cla

Bacterioferritin comigratory protein, BCP

In figure 1 they did a sequence alignment for Cla-BCP with Helicobacter pylori strain J99.

Thioredoxin activity also observed in PMID:20335172, their results were not shown in that experiment but were mentioned.

Figure 8a shows the clear protective nature of CLa-BCP on coiled DNA. Lane 1 was the control group for demonstrating protected (coiled) DNA. Lanes 3 and 4 which contained CLa-BCP show nicked circular (NC) DNA and supercoiled (SC) DNA rather than in lane 2 which showed uncoiled, unprotected, linear DNA from the effects of H2O2.

"The BCP gene (GenBank: ACT56685.1, originally annotated as BCP gene) was amplified by PCR using CLA genomic DNA." This corresponds to CLIBASIA_00485.

Figure 3, part a, shows the peroxiredoxin assay and the significance of CLa-BCP in removing H2O2. There is an indirect relationship between the amount of Cla-BCP and the percentage of disappearance of H2O2 Liberibacter asiaticus" (aka Candidatus Liberibacter asiaticus psy62)