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User:Sarahswartz

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CACAO Spring 2017

My Annotations

StatusPageDate/TimeGO Term (Aspect)ReferenceEvidenceNotesLinks
updatedbyinstructorLIBAP:C6XHE22017-03-23 15:54:49 CDTGO:0051920 peroxiredoxin activity (F)PMID:27987036ECO:0000314 direct assay evidence used in manual assertion

Figure 3, part a, shows the peroxiredoxin assay and the significance of CLa-BCP in removing H2O2. There is an indirect relationship between the amount of Cla-BCP and the percentage of disappearance of H2O2 Liberibacter asiaticus" (aka Candidatus Liberibacter asiaticus psy62)

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updatedbyinstructorLIBAP:C6XHE22017-03-23 16:35:29 CDTGO:0042262 DNA protection (P)PMID:27987036ECO:0000314 direct assay evidence used in manual assertion

Figure 8a shows the clear protective nature of CLa-BCP on coiled DNA. Lane 1 was the control group for demonstrating protected (coiled) DNA. Lanes 3 and 4 which contained CLa-BCP show nicked circular (NC) DNA and supercoiled (SC) DNA rather than in lane 2 which showed uncoiled, unprotected, linear DNA from the effects of H2O2.

"The BCP gene (GenBank: ACT56685.1, originally annotated as BCP gene) was amplified by PCR using CLA genomic DNA." This corresponds to CLIBASIA_00485.

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