Category:Team Blue-A

The authors use a ATPase assay to determine if the helicase D10 can unwind complex branched DNA substrates. Figure 2b shows the ATP dependent unwinding of cruciform (four-way junction) DNA molecules by the T5 helicase gene D10.

GP13 is seen as structural component of the phage ϕ29 tail. Figure 2, uses Western Blot Analysis to infer that gp13 is a component of the tail fiber. Loss of gp13 causes the tail tip, results in a shortened tail (missing tail fibers) . This is specified in Table 3, which quantifies assembly of WT and several gp 13 mutants.

Authors use AFM and Western blotting to analyze the product of gene 44, referred to as G44P, and how it binds to Holliday junction (HJ) structures (Figure 5) then cleaves 1-2 bp away from both static and mobile HJs (Figure 6).

Figures 5 & 6 show binding of gp16 to the procapsid-pRNA complex in vitro, much more so that to procapsid alone, confirming the synergistic role of gp16 and pRNA in the translocation of DNA into the viral procapsid.