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PMID:18394643

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Citation

Cohen, DN, Erickson, SE, Xiang, Y, Rossmann, MG and Anderson, DL (2008) Multifunctional roles of a bacteriophage phi 29 morphogenetic factor in assembly and infection. J. Mol. Biol. 378:804-17

Abstract

Low copy number proteins within macromolecular complexes, such as viruses, can be critical to biological function while comprising a minimal mass fraction of the complex. The Bacillus subtilis double-stranded DNA bacteriophage phi 29 gene 13 product (gp13), previously undetected in the virion, was identified and localized to the distal tip of the tail knob. Western blots and immuno-electron microscopy detected a few copies of gp13 in phi 29, DNA-free particles, purified tails, and defective particles produced in suppressor-sensitive (sus) mutant sus13(330) infections. Particles assembled in the absence of intact gp13 (sus13(342) and sus13(330)) had the gross morphology of phi 29 but were not infectious. gp13 has predicted structural homology and sequence similarity to the M23 metalloprotease LytM. Poised at the tip of the phi 29 tail knob, gp13 may serve as a plug to help restrain the highly pressurized packaged genome. Also, in this position, gp13 may be the first virion protein to contact the cell wall in infection, acting as a pilot protein to depolymerize the cell wall. gp13 may facilitate juxtaposition of the tail knob onto the cytoplasmic membrane and the triggering of genome injection.

Links

PubMed PMC2443984 Online version:10.1016/j.jmb.2008.02.068

Keywords

Amino Acid Motifs; Amino Acid Sequence; Bacillus Phages/genetics; Bacillus Phages/metabolism; Bacillus Phages/ultrastructure; Bacillus subtilis/genetics; Bacillus subtilis/metabolism; Bacillus subtilis/virology; Cryoelectron Microscopy; Microscopy, Electron, Transmission; Microscopy, Immunoelectron; Molecular Sequence Data; Mutation/genetics; Sensitivity and Specificity; Viral Envelope Proteins/chemistry; Viral Envelope Proteins/genetics; Viral Envelope Proteins/isolation & purification; Viral Envelope Proteins/metabolism; Virus Assembly

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

BPPH2:GP13

located_in

GO:0098023: virus tail, tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPH2:VG13

part_of

GO:0098023: virus tail, tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPH2:GP13

GO:0098023: virus tail, tip

ECO:0005592: immunogold labelling electron microscopy assay evidence used in manual assertion

C

Notes: Figure 3 shows that Morphogenesis protein 1 (gp13) in Bacillus phage phi29 is located at the distal end of the ϕ29 tail knob using an Immuno-gold localization.

complete

BPPH2:GP13

part_of

GO:0098023: virus tail, tip

ECO:0000314: direct assay evidence used in manual assertion

C

Seeded From UniProt

complete

BPPH2:GP13

GO:0098004: virus tail fiber assembly

ECO:0000314:

P

GP13 is seen as structural component of the phage ϕ29 tail. Figure 2, uses Western Blot Analysis to infer that gp13 is a component of the tail fiber. Loss of gp13 causes the tail tip, results in a shortened tail (missing tail fibers) . This is specified in Table 3, which quantifies assembly of WT and several gp 13 mutants.

complete
CACAO 12592

BPPH2:GP13

GO:0098015: virus tail

ECO:0000279: western blot evidence used in manual assertion

C

Figure 2 shows that Morphogenesis protein 1 (gp13) in Bacillus phage phi29 is a structural component of the phi 29 tail.

complete
CACAO 13807

Notes

See also

References

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