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BPPH2:PKG16
Contents
| Species (Taxon ID) | Bacillus phage phi29 (Bacteriophage phi-29). (10756) | |
| Gene Name(s) | 16 | |
| Protein Name(s) | DNA packaging protein (ECO:0000303 with PMID:2960820[1])
ATPase gp16 (ECO:0000303 with PMID:23706809[2]) Gene product 16 gp16 Protein p16 | |
| External Links | ||
| UniProt | P11014 | |
| EMBL | M14782 M14431 EU771092 | |
| PIR | B33078 | |
| RefSeq | YP_002004545.1 | |
| PDB | 5HD9 | |
| PDBsum | 5HD9 | |
| SMR | P11014 | |
| GeneID | 6446516 | |
| KEGG | vg:6446516 | |
| OrthoDB | VOG090000DE | |
| Proteomes | UP000001207 | |
| GO | GO:0005524 GO:0016887 GO:0003677 GO:0003723 GO:0019073 GO:0019076 | |
| Gene3D | 3.40.50.300 | |
| InterPro | IPR027417 IPR008784 | |
| Pfam | PF05894 | |
| SUPFAM | SSF52540 | |
Annotations
| Qualifier | GO ID | GO term name | Reference | ECO ID | ECO term name | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|---|---|
|
Contributes to |
GO:0019073 |
viral DNA genome packaging |
ECO:0000314 |
P |
Figures 5 & 6 show binding of gp16 to the procapsid-pRNA complex in vitro, much more so that to procapsid alone, confirming the synergistic role of gp16 and pRNA in the translocation of DNA into the viral procapsid. |
complete | ||||
|
Contributes to |
GO:0019073 |
viral DNA genome packaging |
other:PMC60195 |
ECO:0000314 |
P |
Figure 2 shows that packaged DNA could only be extracted when all components of the packaging system, including p16, were present. Lone RNA, DNA terminal protein, or p16 did not produce packaged DNA. (this article is from pubmed but there was no PMID available.) |
complete | |||
|
enables |
GO:0016887 |
ATPase activity |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
involved_in |
GO:0019073 |
viral DNA genome packaging |
ECO:0000314 |
direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000314 |
direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0005524 |
ATP binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0016787 |
hydrolase activity |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003677 |
DNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0003723 |
RNA binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
|
enables |
GO:0000166 |
nucleotide binding |
ECO:0000322 |
imported manually asserted information used in automatic assertion |
F |
Seeded From UniProt |
complete | |||
Notes
References
See Help:References for how to manage references in GONUTS.
- ↑ Guo, P et al. (1987) Prohead and DNA-gp3-dependent ATPase activity of the DNA packaging protein gp16 of bacteriophage phi 29. J. Mol. Biol. 197 229-36 PubMed GONUTS page
- ↑ 2.0 2.1 Schwartz, C et al. (2013) The ATPase of the phi29 DNA packaging motor is a member of the hexameric AAA+ superfamily. Virology 443 20-7 PubMed GONUTS page
- ↑ Lee, TJ & Guo, P (2006) Interaction of gp16 with pRNA and DNA for genome packaging by the motor of bacterial virus phi29. J. Mol. Biol. 356 589-99 PubMed GONUTS page
- ↑ 4.0 4.1 Koti, JS et al. (2008) DNA packaging motor assembly intermediate of bacteriophage phi29. J. Mol. Biol. 381 1114-32 PubMed GONUTS page
