Category:MichSt14A 2

Figure 3.A Shows the impact of tagO mutants (TagO1, tagO deleted mutants) on the autolytic activity of the cells due to a weaken cell wall . The TagO1 mutants are shown to have a higher percentage of lysis against the wild-type CSF41498 cells. This shows an impact on the synthesis of the cell wall’s integrity as a result of the deletion of the tagO gene.

Figure 2. Shows the changes of optical densities over time for ClpE containing cells; the ClpE genes were transcriptionally fused to either the lacZ or bgaB to measure beta-galactosidase activity. These cells underwent a puromycin treatment to emulate heat shock at time 0 hours. The results show higher expression of ClpE during puromycin treatment.

Figure 3. Shows the absorbance of E.coli cotransformed with plasmids carrying wild-type (BI-95) or mutant dr932 genes; absorbance indicates the production of the red carotenoid. The results show that plasmids containing mutant dr932 genes do not produce the carotenoid but the wildtype BI-95 does.

Figure 4. Shows a polyacrylamide gel of a iscR mutant to test for catalase (KatA) activity. The gel shows a decreased KatA and SodB levels during growth phases, linking iscR to transcriptional regulation of antioxidant enzymes.