User:Bakerk27

My Annotations

Status	Page	Date/Time	GO Term (Aspect)	Reference	Evidence	Notes	Links
acceptable	PSEA7:A6V0U7	2014-04-05 10:19:05 CDT	GO:0006351 transcription, DNA-templated (P)	PMID:18051604	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 4. Shows a polyacrylamide gel of a iscR mutant to test for catalase (KatA) activity. The gel shows a decreased KatA and SodB levels during growth phases, linking iscR to transcriptional regulation of antioxidant enzymes.	challenge
acceptable	DEIRA:Q9RUJ1	2014-04-05 10:38:47 CDT	GO:0008299 isoprenoid biosynthetic process (P)	PMID:24151908	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3. Shows the absorbance of E.coli cotransformed with plasmids carrying wild-type (BI-95) or mutant dr932 genes; absorbance indicates the production of the red carotenoid. The results show that plasmids containing mutant dr932 genes do not produce the carotenoid but the wildtype BI-95 does.	challenge
unacceptable	STRPB:Q1JBK0	2014-04-05 15:08:59 CDT	GO:0000160 phosphorelay signal transduction system (Figure 4.A-C Shows hydrogen peroxide assays of wild-type spy1236 (CiaH) containing cells as well as cells with deleted spy1236 genes. The assay pertains to the cells ability to withstand oxidative damage using hydrogen peroxide treatments,as it is testing spy1236 role in oxidative stress response. Overall, whenever the cell contained the knockout spy1326 gene, there was a lower survivability.)	PMID:24673808	IMP: Inferred from Mutant Phenotype		challenge
unacceptable	BACSU:CLPE	2014-04-06 09:17:48 CDT	GO:0006950 response to stress (P)	PMID:10320580	ECO:0000314 direct assay evidence used in manual assertion	Figure 2. Shows the changes of optical densities over time for ClpE containing cells; the ClpE genes were transcriptionally fused to either the lacZ or bgaB to measure beta-galactosidase activity. These cells underwent a puromycin treatment to emulate heat shock at time 0 hours. The results show higher expression of ClpE during puromycin treatment.	challenge
unacceptable	STAEP:D4FNI2	2014-04-06 10:14:25 CDT	GO:0008963 phospho-N-acetylmuramoyl-pentapeptide-transferase activity (F)	PMID:21051486	ECO:0000315 mutant phenotype evidence used in manual assertion	Figure 3.A Shows the impact of tagO mutants (TagO1, tagO deleted mutants) on the autolytic activity of the cells due to a weaken cell wall . The TagO1 mutants are shown to have a higher percentage of lysis against the wild-type CSF41498 cells. This shows an impact on the synthesis of the cell wall’s integrity as a result of the deletion of the tagO gene.	challenge

acceptable:2
unacceptable:3
requires_changes:0
flagged:0

Annotations challenged by Bakerk27

Status	Author,Group	Page	GO Term (Aspect)	Reference	Evidence	Links	Page history
updatedbyinstructor	Tatkinson, Team Thndrcats Ho!	HUMAN:QSOX1	GO:0016242 - negative regulation of macroautophagy (P)	PMID:24475161	ECO:0000315 mutant phenotype evidence used in manual assertion	challenge	C: 1 fixed by Bakerk27

1 annotations fixed by Bakerk27

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