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PMID:10320580
| Citation |
Derré, I, Rapoport, G, Devine, K, Rose, M and Msadek, T (1999) ClpE, a novel type of HSP100 ATPase, is part of the CtsR heat shock regulon of Bacillus subtilis. Mol. Microbiol. 32:581-93 |
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| Abstract |
Clp ATPases, which include the ubiquitous HSP100 family, are classified according to their structural features and sequence similarities. During the course of the Bacillus subtilis genome sequencing project, we identified a gene encoding a new member of the HSP100 family. We designated this protein ClpE, as it is the prototype of a novel subfamily among the Clp ATPases, and have identified homologues in several bacteria, including Listeria monocytogenes, Enterococcus faecalis, Streptococcus pyogenes, Streptococcus pneumoniae, Lactobacillus sakei and Clostridium acetobutylicum. A unique feature of these Hsp100-type Clp ATPases is their amino-terminal zinc finger motif. Unlike the other class III genes of B. subtilis (clpC and clpP ), clpE does not appear to be required for stress tolerance. Transcriptional analysis revealed two sigmaA-type promoters, expression from which was shown to be inducible by heat shock and puromycin treatment. Investigation of the regulatory mechanism controlling clpE expression indicates that this gene is controlled by CtsR and is thus a member of the class III heat shock genes of B. subtilis. CtsR negatively regulates clpE expression by binding to the promoter region, in which five CtsR binding sites were identified through DNase I footprinting and sequence analysis. |
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| Keywords |
Adenosine Triphosphatases/drug effects; Adenosine Triphosphatases/genetics; Adenosine Triphosphatases/metabolism; Amino Acid Sequence; Anti-Bacterial Agents/pharmacology; Bacillus subtilis/drug effects; Bacillus subtilis/physiology; Bacterial Proteins/drug effects; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Gene Expression Regulation, Bacterial/drug effects; Heat-Shock Proteins/drug effects; Heat-Shock Proteins/genetics; Heat-Shock Proteins/metabolism; Heat-Shock Response; Molecular Chaperones/metabolism; Molecular Sequence Data; Mutation; Promoter Regions, Genetic; Protozoan Proteins/genetics; Protozoan Proteins/metabolism; Puromycin/pharmacology; Repressor Proteins/genetics; Repressor Proteins/metabolism; Sequence Homology, Amino Acid |
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Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0006950: response to stress |
ECO:0000314: |
P |
Figure 2. Shows the changes of optical densities over time for ClpE containing cells; the ClpE genes were transcriptionally fused to either the lacZ or bgaB to measure beta-galactosidase activity. These cells underwent a puromycin treatment to emulate heat shock at time 0 hours. The results show higher expression of ClpE during puromycin treatment. |
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